Details:

Alternative Title:
Clin Infect Dis
Personal Author:
McGee, Lesley ; Chochua, Sopio ; Li, Zhongya ; Mathis, Saundra ; Rivers, Joy ; ... More +
McGee, Lesley ; Chochua, Sopio ; Li, Zhongya ; Mathis, Saundra ; Rivers, Joy ; Metcalf, Benjamin ; Ryan, Alison ; Alden, Nisha ; Farley, Monica M. ; Harrison, Lee H. ; Snippes Vagnone, Paula ; Lynfield, Ruth ; Smelser, Chad ; Muse, Alison ; Thomas, Ann. R ; Schrag, Stephanie ; Beall, Bernard W. Less -
Description:
Background:

Group B Streptococcus (GBS) is a leading cause of neonatal sepsis and meningitis and an important cause of invasive infections in pregnant and nonpregnant adults. Vaccines targeting capsule polysaccharides and common proteins are under development.

Methods:

Using whole genome sequencing (WGS), a validated bioinformatics pipeline, and targeted antimicrobial susceptibility testing, we characterized 6,340 invasive GBS isolates recovered during 2015–2017 through population-based Active Bacterial Core surveillance (ABCs) in eight states.

Results:

Six serotypes accounted for 98.4% of isolates (21.8% Ia, 17.6% V, 17.1% II, 15.6% III, 14.5% Ib, 11.8% IV). Most (> 98%) of these six serotypes were in seven clonal complexes (CCs) comprised of multilocus sequence types (MLSTs) identical or closely related to STs 1, 12, 17, 19, 22, 23, and 459. Fifty-four isolates (0.87%) had point mutations within pbp2x (31 different alleles) associated with reduced susceptibility to one or more β-lactam antibiotics. Genes conferring resistance to macrolides and/or lincosamides were found in 56% of isolates; 85.2% of isolates had tetracycline resistance genes. Two isolates carrying vanG were vancomycin-nonsusceptible (MIC 2μg/ml). Nearly all isolates possessed capsule genes, 1–2 of the three main pilus gene clusters (or islands), and one of four homologous Alpha/Rib family determinants. Presence of hvgA virulence gene was primarily restricted to serotype III/CC17 isolates (465 isolates), but 8 exceptions (7 IV/CC23 and 1 IV/CC17) were observed.

Conclusions:

This first comprehensive, population-based quantitation of strain features in the United States suggests current vaccine candidates should have good coverage. Beta-lactams remain appropriate for first line treatment and prophylaxis, but emergence of nonsusceptibility warrants ongoing monitoring.


Subjects:
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Adult Anti-Bacterial Agents Drug Resistance, Bacterial Female Genotype Humans Microbial Sensitivity Tests Pregnancy Serogroup Serotyping Streptococcal Infections Streptococcus Agalactiae Vaccines
Source:
Clin Infect Dis. 72(6):1004-1013
Pubmed ID:
32060499
Pubmed Central ID:
PMC8071603
Document Type:
Journal Article
Funding:
WT_/Wellcome TrustUnited Kingdom/ ; CC999999/ImCDC/Intramural CDC HHSUnited States/
WT_/Wellcome TrustUnited Kingdom/ ; CC999999/ImCDC/Intramural CDC HHSUnited States/ Less -
Place as Subject:
United States
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:733d1516ab1afe00552313c37cf8308524da442a22c7910976193d7eb41f5e08
Download URL:
https://stacks.cdc.gov/view/cdc/105566/cdc_105566_DS1.pdf
File Type:

Supporting Files

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