Comparative Omics Analysis of Historic and Recent Isolates of Bordetella pertussis and Effects of Genome Rearrangements on Evolution

Dienstbier, Ana; Amman, Fabian; Petráčková, Denisa; Štipl, Daniel; Čapek, Jan; Zavadilová, Jana; Fabiánová, Kateřina; Držmíšek, Jakub; Kumar, Dilip; Wildung, Mark; Pouchnik, Derek; Večerek, Branislav

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Comparative Omics Analysis of Historic and Recent Isolates of Bordetella pertussis and Effects of Genome Rearrangements on Evolution

Supporting Files Public Domain

Jan 2021
By Dienstbier, Ana ; Amman, Fabian ; Petráčková, Denisa ; ...

File Language:

English

Details

Alternative Title:

Emerg Infect Dis
Personal Author:

Dienstbier, Ana ; Amman, Fabian ; Petráčková, Denisa ; Štipl, Daniel ; Čapek, Jan ; Zavadilová, Jana ; Fabiánová, Kateřina ; Držmíšek, Jakub ; Kumar, Dilip ; Wildung, Mark ; Pouchnik, Derek ; Večerek, Branislav
Description:

Despite high vaccination coverage, pertussis is increasing in many industrialized countries, including the Czech Republic. To better understand Bordetella pertussis resurgence, we analyzed historic strains and recent clinical isolates by using a comparative omics approach. Whole-genome sequencing showed that historic and recent isolates of B. pertussis have substantial variation in genome organization and form separate phylogenetic clusters. Subsequent RNA sequence analysis and liquid chromatography with mass tandem spectrometry analyses showed that these variations translated into discretely separated transcriptomic and proteomic profiles. When compared with historic strains, recent isolates showed increased expression of flagellar genes and genes involved in lipopolysaccharide biosynthesis and decreased expression of polysaccharide capsule genes. Compared with reference strain Tohama I, all strains had increased expression and production of the type III secretion system apparatus. We detected the potential link between observed effects and insertion sequence element-induced changes in gene context only for a few genes.
Subjects:

Bacteria Bordetella Pertussis Comparative Omics Analysis Of Historic And Recent Isolates Of Bordetella Pertussis And Effects Of Genome Rearrangements On Evolution Czech Republic Genomic Rearrangements Insertion Sequence Element Omics Analysis Pathogen Evolution Pertussis Research Whooping Cough
Source:

Emerg Infect Dis. 27(1):57-68
Pubmed ID:

33350934
Pubmed Central ID:

PMC7774529
Document Type:

Journal Article
Volume:

27
Issue:

1
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:6853fb9ae1bf795d123e8ecea2990c9ad44f0bc6e1a9e188f88b6649e310b644
Download URL:

https://stacks.cdc.gov/view/cdc/99965/cdc_99965_DS1.pdf
File Type:

[PDF - 3.00 MB ]

File Language:

English

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