Details:

Alternative Title:
Rapid Commun Mass Spectrom
Personal Author:
De Jesús, Víctor R. ; Silva, Lalith K. ; Newman, Cody A. ; Blount, Benjamin C.
De Jesús, Víctor R. ; Silva, Lalith K. ; Newman, Cody A. ; Blount, Benjamin C. Less -
Description:
Rationale:

Over 2,800 e-cigarette, or vaping, product use-associated lung injury (EVALI) cases have been reported to the Centers for Disease Control and Prevention (CDC) during August 2019-February 2020. Bronchoalveolar lavage (BAL) fluid samples from 51 EVALI and 99 non-EVALI cases were analyzed for toxicants including terpenes. We describe a novel method to measure selected terpenes in BAL fluid by gas chromatography-tandem mass spectrometry (GC/MS/MS).

Methods:

α-Pinene, ß-pinene, ß-myrcene, 3-carene, and limonene were measured in BAL fluid specimens by headspace solid-phase microextraction/gas chromatography/tandem mass spectrometry. We created and characterized BAL fluid pools from non-EVALI individuals to determine assay accuracy, precision, linearity, limits of detection, and analytical specificity. All measurements were conducted in accordance with the CDC’s Division of Laboratory Sciences rigorous method validation procedures.

Results:

Matrix validation experiments showed that calibration curves in BAL fluid and saline had similar slopes, with differences less than 7%. Assay precision ranged from 2.52% - 5.30%. In addition, the limits of detection for the five analytes ranged from 1.80 – 16.8 ng/L, and their linearity was confirmed with R2 values >0.99.

Conclusions:

We developed and validated a method to quantify selected terpenes in BAL fluid specimens using GC/MS/MS. The assay provided accurate and precise analyses of EVALI and non-EVALI BAL fluid specimens in support of CDC’s EVALI response. This method is applicable to the determination of a broad range of terpenes in BAL fluid specimens.


Subjects:
[+]
Article Bronchoalveolar Lavage Fluid EVALI Gas Chromatography-Mass Spectrometry GC/MS/MS Humans Limit Of Detection Linear Models Lung Injury Monoterpenes Reproducibility Of Results Solid Phase Microextraction Terpenes Vaping
Source:
Rapid Commun Mass Spectrom. 34(19):e8879
Pubmed ID:
32632930
Pubmed Central ID:
PMC7719058
Document Type:
Journal Article
Funding:
CC999999/ImCDC/Intramural CDC HHSUnited States/
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:33fb4fea6f7aac98e9ec614d76c23043556f3ba1f0c959d53a45b593926ee9dd
Download URL:
https://stacks.cdc.gov/view/cdc/98484/cdc_98484_DS1.pdf
File Type:

Supporting Files

• 	nihms-1617765-f0001.gif	gif
  	nihms-1617765-f0001.jpg	jpeg
  	nihms-1617765-f0002.gif	gif
  	nihms-1617765-f0002.jpg	jpeg
  	nihms-1617765.nxml	xml

More +