Liquid chromatography-tandem mass spectrometry method for measuring vitamin E acetate in bronchoalveolar lavage fluid

Details

• Alternative Title:
  J Chromatogr B Analyt Technol Biomed Life Sci
• Personal Author:
  Espinosa, Maria Morel ; Blount, Benjamin C. ; Valentin-Blasini, Liza
• Description:
  We investigated the suitability of isotope-dilution liquid chromatography coupled with tandem mass spectrometry for identifying vitamin E acetate (VEA) in bronchoalveolar lavage (BAL) fluid. This new method demonstrates high accuracy, selectivity, and sensitivity, with mean recoveries higher than 90%, coefficients of variation ranging from 1.5% to 4.5%, and a limit of detection of 1.10 ng/mL. Calibration curves were linear (R| > 0.99). The linear range and detection limit of the method were adequate for identifying VEA in 48 of 51 BAL fluid samples collected from people with lung injury resulting from e-cigarettes, or vaping, product use. We conclude that this method is an effective tool for studying VEA accumulation in lungs caused by using e-cigarettes, or vaping, products that contain VEA.
• Subjects:
  Alpha-Tocopherol Bronchoalveolar Lavage Fluid Chromatography, High Pressure Liquid Electronic Nicotine Delivery Systems Humans Limit Of Detection Linear Models Lung Injury Reproducibility Of Results Tandem Mass Spectrometry Vaping

  More +
• Keywords:
  Bronchoalveolar Lavage Fluid Isotope Dilution LC-MS/MS Vitamin E Acetate
• Source:
  J Chromatogr B Analyt Technol Biomed Life Sci. 1171:122607
• Pubmed ID:
  33740693
• Pubmed Central ID:
  PMC8046728
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  1171
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:361cdf0484192732dce3e0288da2367d30bde19e1073b9a0c69d924d48d68557
• Download URL:
  https://stacks.cdc.gov/view/cdc/105279/cdc_105279_DS1.pdf
• File Type:
  [PDF - 410.83 KB ]