Validated Methods for Removing Select Agent Samples from Biosafety Level 3 Laboratories

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Kesterson, Alexandria E. ; Craig, John E. ; Chuvala, Lara J. ; Heine, Henry S.
• Description:
  The Federal Select Agent Program dictates that all research entities in the United States must rigorously assess laboratory protocols to sterilize samples being removed from containment areas. We validated procedures using sterile filtration and methanol to remove the following select agents: Francisella tularensis, Burkholderia pseudomallei, B. mallei, Yersinia pestis, and Bacillus anthracis. We validated methanol treatment for B. pseudomallei. These validations reaffirm safety protocols that enable researchers to keep samples sufficiently intact when samples are transferred between laboratories.
• Subjects:
  Bacillus Anthracis Bacteria Biosafety Level 3 BSL-3 Burkholderia Mallei Burkholderia Pseudomallei Filtration Francisella Tularensis Methanol Research Sample Removal Select Agents Sterility Validated Methods For Removing Select Agent Samples From Biosafety Level 3 Laboratories Validation Yersinia Pestis

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• Source:
  Emerg Infect Dis. 26(11):2586-2590
• Pubmed ID:
  33079040
• Pubmed Central ID:
  PMC7588557
• Document Type:
  Journal Article
• Volume:
  26
• Issue:
  11
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:c59ae7a0ece11e254ede55e72b017379a6e08329d574bcc0778974df8b489485
• Download URL:
  https://stacks.cdc.gov/view/cdc/96897/cdc_96897_DS1.pdf
• File Type:
  [PDF - 704.37 KB ]