Pooling Upper Respiratory Specimens for Rapid Mass Screening of COVID-19 by Real-Time RT-PCR

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Kim, So Yeon ; Lee, Jaehyeon ; Sung, Heungsup ; Lee, Hyukmin ; Han, Myung Guk ; Yoo, Cheon Kwon ; Lee, Sang Won ; Hong, Ki Ho
• Description:
  To validate the specimen-pooling strategy for real-time reverse transcription PCR detection of severe acute respiratory syndrome coronavirus 2, we generated different pools including positive specimens, reflecting the distribution of cycle threshold values at initial diagnosis. Cumulative sensitivities of tested pool sizes suggest pooling of <6 specimens for surveillance by this method.
• Subjects:
  Betacoronavirus Clinical Laboratory Techniques Coronavirus Infections Covid-19 Humans Mass Screening Nasopharynx Oropharynx Pandemics Pneumonia, Viral Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Sensitivity And Specificity

  More +
• Source:
  Emerg Infect Dis. 26(10):2469-2472
• Pubmed ID:
  32844739
• Pubmed Central ID:
  PMC7510748
• Document Type:
  Journal Article
• Place as Subject:
  Republic of Korea
• Location:
  Asia
• Volume:
  26
• Issue:
  10
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:d86aa71ee7b438ab6422613e6039271ca32ebd8f83cc2ffe05483e9dabdf7f9e
• Download URL:
  https://stacks.cdc.gov/view/cdc/94774/cdc_94774_DS1.pdf
• File Type:
  [PDF - 635.45 KB ]