Crass phage as a Novel Tool to Detect Human Fecal Contamination on Environmental Surfaces and Hands

Park, Geun Woo; Ng, Terry Fei Fan; Freeland, Amy L.; Marconi, Vincent C.; Boom, Julie A.; Staat, Mary A.; Montmayeur, Anna Maria; Browne, Hannah; Narayanan, Jothikumar; Payne, Daniel C.; Cardemil, Cristina V.; Treffiletti, Aimee; Vinjé, Jan

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Crass phage as a Novel Tool to Detect Human Fecal Contamination on Environmental Surfaces and Hands

Supporting Files Public Domain

June 08 2020
By Park, Geun Woo ; Ng, Terry Fei Fan ; Freeland, Amy L. ; ...

File Language:

English

Details

Alternative Title:

Emerg Infect Dis
Personal Author:

Park, Geun Woo ; Ng, Terry Fei Fan ; Freeland, Amy L. ; Marconi, Vincent C. ; Boom, Julie A. ; Staat, Mary A. ; Montmayeur, Anna Maria ; Browne, Hannah ; Narayanan, Jothikumar ; Payne, Daniel C. ; Cardemil, Cristina V. ; Treffiletti, Aimee ; Vinjé, Jan
Description:

CrAssphage is a recently discovered human gut-associated bacteriophage. To validate the potential use of crAssphage for detecting human fecal contamination on environmental surfaces and hands, we tested stool samples (n = 60), hand samples (n = 30), and environmental swab samples (n = 201) from 17 norovirus outbreaks for crAssphage by real-time PCR. In addition, we tested stool samples from healthy persons (n = 173), respiratory samples (n = 113), and animal fecal specimens (n = 68) and further sequenced positive samples. Overall, we detected crAssphage in 71.4% of outbreak stool samples, 48%-68.5% of stool samples from healthy persons, 56.2% of environmental swabs, and 60% of hand rinse samples, but not in human respiratory samples or animal fecal samples. CrAssphage sequences could be grouped into 2 major genetic clusters. Our data suggest that crAssphage could be used to detect human fecal contamination on environmental surfaces and hands.
Subjects:

CrAssphage CrAssphage As A Novel Tool To Detect Human Fecal Contamination On Environmental Surfaces And Hands Cruise Ship Enteric Infections Environmental Contamination Hand Contamination Human Fecal Indicator Long-term Care Facility Norovirus Outbreak Research Viruses
Source:

Emerg Infect Dis. 26(8):1731-1739
Pubmed ID:

32511090
Pubmed Central ID:

PMC7392416
Document Type:

Journal Article
Volume:

26
Issue:

8
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:2fd9b61207cecba1e21d3ee1a808181528ac999e9cc4ef9d37300f56b160c7fd
Download URL:

https://stacks.cdc.gov/view/cdc/91553/cdc_91553_DS1.pdf
File Type:

[PDF - 2.95 MB ]

File Language:

English

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