Identification of novel respiratory syncytial virus CD4+ and CD8+ T cell epitopes in C57BL/6 mice

Details

• Alternative Title:
  Immunohorizons
• Personal Author:
  Schmidt, Megan E. ; Varga, Steven M.
• Description:
  Respiratory syncytial virus (RSV) is the most common cause of lower respiratory tract infection and hospitalization in infants. It is well established that both CD4| and CD8| T cells are critical for mediating viral clearance but also contribute to the induction of immunopathology following RSV infection. C57BL/6 mice are often used to study T cell responses following RSV infection given the wide variety of genetically modified animals available. To date, few RSV-derived CD4| and CD8| T cell epitopes have been identified in C57BL/6 mice. Using an overlapping peptide library spanning the entire RSV proteome, intracellular cytokine staining for IFN-γ was performed to identify novel CD4| and CD8| T cell epitopes in C57BL/6 mice. We identified two novel CD4| T cell epitopes and three novel CD8| T cell epitopes located within multiple RSV proteins. Additionally, we characterized the newly described T cell epitopes by determining their TCR Vβ expression profiles and MHC restriction. Overall, the novel RSV-derived CD4| and CD8| T cell epitopes identified in C57BL/6 mice will aid in future studies of RSV-specific T cell responses.
• Subjects:
  Animals Article CD4-Positive T-Lymphocytes CD8-Positive T-Lymphocytes Cell Line, Tumor Epitopes, T-Lymphocyte Female Histocompatibility Antigens Class I Interferon-gamma Lung Mice Mice, Inbred C57BL Peptide Library Receptors, Antigen, T-Cell, Alpha-beta Respiratory Syncytial Virus, Human Respiratory Syncytial Virus Infections Viral Proteins

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• Source:
  Immunohorizons. 3(1):1-12
• Pubmed ID:
  31356172
• Pubmed Central ID:
  PMC7316092
• Document Type:
  Journal Article
• Funding:
  R01 AI124093/AI/NIAID NIH HHS/United States ; S10 OD016199/OD/NIH HHS/United States ; T32 AI007485/AI/NIAID NIH HHS/United States ; S10 OD016199/CD/ODCDC CDC HHS/United States
• Volume:
  3
• Issue:
  1
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:c8d65cdf212c610466c071ec58ea45a5fd5ff71772b865ada21b902d4601282a
• Download URL:
  https://stacks.cdc.gov/view/cdc/89865/cdc_89865_DS1.pdf
• File Type:
  [PDF - 1.29 MB ]