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Identification of novel respiratory syncytial virus CD4+ and CD8+ T cell epitopes in C57BL/6 mice
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January 14 2019
Source: Immunohorizons. 3(1):1-12
[PDF-1.29 MB]
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Alternative Title:Immunohorizons
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Description:Respiratory syncytial virus (RSV) is the most common cause of lower respiratory tract infection and hospitalization in infants. It is well established that both CD4| and CD8| T cells are critical for mediating viral clearance but also contribute to the induction of immunopathology following RSV infection. C57BL/6 mice are often used to study T cell responses following RSV infection given the wide variety of genetically modified animals available. To date, few RSV-derived CD4| and CD8| T cell epitopes have been identified in C57BL/6 mice. Using an overlapping peptide library spanning the entire RSV proteome, intracellular cytokine staining for IFN-γ was performed to identify novel CD4| and CD8| T cell epitopes in C57BL/6 mice. We identified two novel CD4| T cell epitopes and three novel CD8| T cell epitopes located within multiple RSV proteins. Additionally, we characterized the newly described T cell epitopes by determining their TCR Vβ expression profiles and MHC restriction. Overall, the novel RSV-derived CD4| and CD8| T cell epitopes identified in C57BL/6 mice will aid in future studies of RSV-specific T cell responses.
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Pubmed ID:31356172
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Pubmed Central ID:PMC7316092
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