Host-Species Transferrin Receptor 1 Orthologs Are Cellular Receptors for Nonpathogenic New World Clade B Arenaviruses

Details

• Alternative Title:
  PLoS Pathog
• Personal Author:
  Abraham, Jonathan ; Kwong, Jo Ann ; Albariño, César G. ; Lu, Jiajie G. ; Radoshitzky, Sheli R. ; Salazar-Bravo, Jorge ; Farzan, Michael ; Spiropoulou, Christina F. ; Choe, Hyeryun
• Description:
  The ability of a New World (NW) clade B arenavirus to enter cells using human transferrin receptor 1 (TfR1) strictly correlates with its ability to cause hemorrhagic fever. Amapari (AMAV) and Tacaribe (TCRV), two nonpathogenic NW clade B arenaviruses that do not use human TfR1, are closely related to the NW arenaviruses that cause hemorrhagic fevers. Here we show that pseudotyped viruses bearing the surface glycoprotein (GP) of AMAV or TCRV can infect cells using the TfR1 orthologs of several mammalian species, including those of their respective natural hosts, the small rodent Neacomys spinosus and the fruit bat Artibeus jamaicensis. Mutation of one residue in human TfR1 makes it a functional receptor for TCRV, and mutation of four residues makes it a functional receptor for AMAV. Our data support an in vivo role for TfR1 in the replication of most, if not all, NW clade B arenaviruses, and suggest that with modest changes in their GPs the nonpathogenic arenaviruses could use human TfR1 and emerge as human pathogens.
• Subjects:
  Amino Acid Sequence Animals Antigens, CD Arenaviruses, New World Arvicolinae Cats Cell Line Chiroptera CHO Cells Cricetinae Cricetulus Dogs Humans Membrane Glycoproteins Mice Molecular Sequence Data Mutagenesis, Site-Directed Phylogeny Rats Receptors, Transferrin Sequence Alignment Species Specificity Viral Proteins Virus Attachment

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• Source:
  PLoS Pathog. 2009; 5(4)
• Pubmed ID:
  19343214
• Pubmed Central ID:
  PMC2658809
• Document Type:
  Journal Article
• Funding:
  R01 AI07487/AI/NIAID NIH HHS/United States ; T32 AI007487/AI/NIAID NIH HHS/United States ; T32 GM007753/GM/NIGMS NIH HHS/United States ; R01 AI074879/AI/NIAID NIH HHS/United States ; Howard Hughes Medical Institute/United States
• Volume:
  5
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:1db6293fd1d9399ead656dfec265d9cc9f6f9490fa475427c4ebf009ce90c933
• Download URL:
  https://stacks.cdc.gov/view/cdc/89857/cdc_89857_DS1.pdf
• File Type:
  [PDF - 892.21 KB ]