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FluChip‐8G Insight: HA and NA subtyping of potentially pandemic influenza A viruses in a single assay
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October 13 2019
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Source: Influenza Other Respir Viruses. 14(1):55-60
[PDF-337.04 KB]
Details:
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Alternative Title:Influenza Other Respir Viruses
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Personal Author:
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Description:Background
Global influenza surveillance in humans and animals is a critical component of pandemic preparedness. The FluChip‐8G Insight assay was developed to subtype both seasonal and potentially pandemic influenza viruses in a single assay with a same day result. FluChip‐8G Insight uses whole gene segment RT‐PCR‐based amplification to provide robustness against genetic drift and subsequent microarray detection with artificial neural network‐based data interpretation.
Objectives
The objective of this study was to verify and validate the performance of the FluChip‐8G Insight assay for the detection and positive identification of human and animal origin non‐seasonal influenza A specimens.
Methods
We evaluated the ability of the FluChip‐8G Insight technology to type and HA and NA subtype a sample set consisting of 297 results from 180 unique non‐seasonal influenza A strains (49 unique subtypes).
Results
FluChip‐8G Insight demonstrated a positive percent agreement ≥93% for 5 targeted HA and 5 targeted NA subtypes except for H9 (88%), and negative percent agreement exceeding 95% for all targeted subtypes.
Conclusions
The FluChip‐8G Insight neural network‐based algorithm used for virus identification performed well over a data set of 297 naïve sample results, and can be easily updated to improve performance on emerging strains without changing the underlying assay chemistry.
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Pubmed ID:31608599
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Pubmed Central ID:PMC6928037
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Funding:R43/R44 AI077112/NIH/NIAID SBIR program/International ; HHSO100201400010C/Department of Health and Human Services; Office of the Assistant Secretary for Preparedness and Response; Biomedical Advanced Research and Development Authority/International ; HHSO100201500024C/Department of Health and Human Services; Office of the Assistant Secretary for Preparedness and Response; Biomedical Advanced Research and Development Authority/International
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