FluChip‐8G Insight: HA and NA subtyping of potentially pandemic influenza A viruses in a single assay

Details

• Alternative Title:
  Influenza Other Respir Viruses
• Personal Author:
  Toth, Evan ; Dawson, Erica D. ; Taylor, Amber W. ; Stoughton, Robert S. ; Blair, Rebecca H. ; Johnson, James E. ; Slinskey, Amelia ; Fessler, Ryan ; Smith, Catherine B. ; Talbot, Sarah ; Rowlen, Kathy
• Description:
  Background
  
  Global influenza surveillance in humans and animals is a critical component of pandemic preparedness. The FluChip‐8G Insight assay was developed to subtype both seasonal and potentially pandemic influenza viruses in a single assay with a same day result. FluChip‐8G Insight uses whole gene segment RT‐PCR‐based amplification to provide robustness against genetic drift and subsequent microarray detection with artificial neural network‐based data interpretation.
  
  Objectives
  
  The objective of this study was to verify and validate the performance of the FluChip‐8G Insight assay for the detection and positive identification of human and animal origin non‐seasonal influenza A specimens.
  
  Methods
  
  We evaluated the ability of the FluChip‐8G Insight technology to type and HA and NA subtype a sample set consisting of 297 results from 180 unique non‐seasonal influenza A strains (49 unique subtypes).
  
  Results
  
  FluChip‐8G Insight demonstrated a positive percent agreement ≥93% for 5 targeted HA and 5 targeted NA subtypes except for H9 (88%), and negative percent agreement exceeding 95% for all targeted subtypes.
  
  Conclusions
  
  The FluChip‐8G Insight neural network‐based algorithm used for virus identification performed well over a data set of 297 naïve sample results, and can be easily updated to improve performance on emerging strains without changing the underlying assay chemistry.
  
  
• Subjects:
  DNA Primers Hemagglutinin Glycoproteins, Influenza Virus Humans Influenza, Human Influenza A Virus Neuraminidase Pandemics Reverse Transcriptase Polymerase Chain Reaction Viral Proteins
• Source:
  Influenza Other Respir Viruses. 14(1):55-60
• Pubmed ID:
  31608599
• Pubmed Central ID:
  PMC6928037
• Document Type:
  Journal Article
• Funding:
  R43/R44 AI077112/NIH/NIAID SBIR program/International ; HHSO100201400010C/Department of Health and Human Services; Office of the Assistant Secretary for Preparedness and Response; Biomedical Advanced Research and Development Authority/International ; HHSO100201500024C/Department of Health and Human Services; Office of the Assistant Secretary for Preparedness and Response; Biomedical Advanced Research and Development Authority/International
• Place as Subject:
  United States
• Volume:
  14
• Issue:
  1
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:cf5992167da91031765ca24db25b660886f80f680c2628a55123d6cf1bd92c4f
• Download URL:
  https://stacks.cdc.gov/view/cdc/89368/cdc_89368_DS1.pdf
• File Type:
  [PDF - 337.04 KB ]