FluChip‐8G Insight: HA and NA subtyping of potentially pandemic influenza A viruses in a single assay

Details

• Alternative Title:
  Influenza Other Respir Viruses
• Personal Author:
  Toth, Evan ; Dawson, Erica D. ; Taylor, Amber W. ; Stoughton, Robert S. ; Blair, Rebecca H. ; Johnson, James E. ; Slinskey, Amelia ; Fessler, Ryan ; Smith, Catherine B. ; Talbot, Sarah ; Rowlen, Kathy
  More +
• Description:
  Background ; Global influenza surveillance in humans and animals is a critical component of pandemic preparedness. The FluChip‐8G Insight assay was developed to subtype both seasonal and potentially pandemic influenza viruses in a single assay with a same day result. FluChip‐8G Insight uses whole gene segment RT‐PCR‐based amplification to provide robustness against genetic drift and subsequent microarray detection with artificial neural network‐based data interpretation. ; Objectives ; The objective of this study was to verify and validate the performance of the FluChip‐8G Insight assay for the detection and positive identification of human and animal origin non‐seasonal influenza A specimens. ; Methods ; We evaluated the ability of the FluChip‐8G Insight technology to type and HA and NA subtype a sample set consisting of 297 results from 180 unique non‐seasonal influenza A strains (49 unique subtypes). ; Results ; FluChip‐8G Insight demonstrated a positive percent agreement ≥93% for 5 targeted HA and 5 targeted NA subtypes except for H9 (88%), and negative percent agreement exceeding 95% for all targeted subtypes. ; Conclusions ; The FluChip‐8G Insight neural network‐based algorithm used for virus identification performed well over a data set of 297 naïve sample results, and can be easily updated to improve performance on emerging strains without changing the underlying assay chemistry.
• Subjects:
  • DNA Primers
  • Hemagglutinin Glycoproteins, Influenza Virus
  • Humans
  • Influenza A virus
  • Influenza, Human
  • Neuraminidase
  • Pandemics
  • Reverse Transcriptase Polymerase Chain Reaction
  • Viral Proteins
• Source:
  Influenza Other Respir Viruses. 14(1):55-60
• Pubmed ID:
  31608599
• Pubmed Central ID:
  PMC6928037
• Document Type:
  Journal Article
• Funding:
  R43/R44 AI077112/NIH/NIAID SBIR program/International ; HHSO100201400010C/Department of Health and Human Services; Office of the Assistant Secretary for Preparedness and Response; Biomedical Advanced Research and Development Authority/International ; HHSO100201500024C/Department of Health and Human Services; Office of the Assistant Secretary for Preparedness and Response; Biomedical Advanced Research and Development Authority/International
• Place as Subject:
  United States
• Volume:
  14
• Issue:
  1
• Download URL:
  https://stacks.cdc.gov/view/cdc/89368/cdc_89368_DS1.pdf
• File Type:
  [PDF - 337.04 KB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:cf5992167da91031765ca24db25b660886f80f680c2628a55123d6cf1bd92c4f