Zika Virus Surveillance at the Human–Animal Interface in West-Central Brazil, 2017–2018

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• Alternative Title:
  Viruses
• Personal Author:
  Pauvolid-Corrêa, Alex ; Gonçalves Dias, Helver ; Marina Siqueira Maia, Laura ; Porfírio, Grasiela ; Oliveira Morgado, Thais ; Sabino-Santos, Gilberto ; Helena Santa Rita, Paula ; Teixeira Gomes Barreto, Wanessa ; Carvalho de Macedo, Gabriel ; Marinho Torres, Jaire ; Arruda Gimenes Nantes, Wesley ; Martins Santos, Filipe ; Oliveira de Assis, William ; Castro Rucco, Andreza ; Mamoru dos Santos Yui, Rafael ; Bosco Vilela Campos, João ; Rodrigues Leandro e Silva, Renato ; da Silva Ferreira, Raquel ; Aparecido da Silva Neves, Nilvanei ; Charlles de Souza Costa, Michell ; Ramos Martins, Leticia ; Marques de Souza, Emerson ; dos Santos Carvalho, Michellen ; Gonçalves Lima, Marina ; de Cássia Gonçalves Alves, Fernanda ; Humberto Guimarães Riquelme-Junior, Luiz ; Luiz Batista Figueiró, Luan ; Fernandes Gomes de Santana, Matheus ; Gustavo Rodrigues Oliveira Santos, Luiz ; Serra Medeiros, Samara ; Lopes Seino, Larissa ; Hime Miranda, Emily ; Henrique Rezende Linhares, José ; de Oliveira Santos, Vanessa ; Almeida da Silva, Stephanie ; Araújo Lúcio, Kelly ; Silva Gomes, Viviane ; de Araújo Oliveira, Alexandre ; dos Santos Silva, Julia ; de Almeida Marques, William ; Schafer Marques, Marcio ; Junior França de Barros, José ; Campos, Letícia ; Couto-Lima, Dinair ; Coutinho Netto, Claudia ; Strüssmann, Christine ; Panella, Nicholas ; Hannon, Emily ; Cristina de Macedo, Barbara ; Ramos de Almeida, Júlia ; Ramos Ribeiro, Karen ; Carolina Barros de Castro, Maria ; Pratta Campos, Larissa ; Paula Rosa dos Santos, Ana ; Marino de Souza, Isabelle ; de Assis Bianchini, Mateus ; Helena Ramiro Correa, Sandra ; Ordones Baptista Luz, Renato ; dos Santos Vieira, Ananda ; Maria de Oliveira Pinto, Luzia ; Azeredo, Elzinandes ; Tadeu Moraes Figueiredo, Luiz ; Augusto Fonseca Alencar, Jeronimo ; Maria Barbosa de Lima, Sheila ; Miraglia Herrera, Heitor ; Dezengrini Shlessarenko, Renata ; Barreto dos Santos, Flavia ; Maria Bispo de Filippis, Ana ; Salyer, Stephanie ; Montgomery, Joel ; Komar, Nicholas
• Description:
  Zika virus (ZIKV) was first discovered in 1947 in Uganda but was not considered a public health threat until 2007 when it found to be the source of epidemic activity in Asia. Epidemic activity spread to Brazil in 2014 and continued to spread throughout the tropical and subtropical regions of the Americas. Despite ZIKV being zoonotic in origin, information about transmission, or even exposure of non-human vertebrates and mosquitoes to ZIKV in the Americas, is lacking. Accordingly, from February 2017 to March 2018, we sought evidence of sylvatic ZIKV transmission by sampling whole blood from approximately 2000 domestic and wild vertebrates of over 100 species in West-Central Brazil within the active human ZIKV transmission area. In addition, we collected over 24,300 mosquitoes of at least 17 genera and 62 species. We screened whole blood samples and mosquito pools for ZIKV RNA using pan-flavivirus primers in a real-time reverse-transcription polymerase chain reaction (RT-PCR) in a SYBR Green platform. Positives were confirmed using ZIKV-specific envelope gene real-time RT-PCR and nucleotide sequencing. Of the 2068 vertebrates tested, none were ZIKV positive. Of the 23,315 non-engorged mosquitoes consolidated into 1503 pools tested, 22 (1.5%) with full data available showed some degree of homology to insect-specific flaviviruses. To identify previous exposure to ZIKV, 1498 plasma samples representing 62 species of domestic and sylvatic vertebrates were tested for ZIKV-neutralizing antibodies by plaque reduction neutralization test (PRNT|). From these, 23 (1.5%) of seven species were seropositive for ZIKV and negative for dengue virus serotype 2, yellow fever virus, and West Nile virus, suggesting potential monotypic reaction for ZIKV. Results presented here suggest no active transmission of ZIKV in non-human vertebrate populations or in alternative vector candidates, but suggest that vertebrates around human populations have indeed been exposed to ZIKV in West-Central Brazil.
• Subjects:
  Article Culicidae Geography, Medical Humans Mosquito Vectors Neutralization Tests Public Health Surveillance Seroepidemiologic Studies Zika Virus Zika Virus Infection Zoonoses

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• Source:
  Viruses. 2019; 11(12)
• Document Type:
  Journal Article
• Place as Subject:
  Brazil
• Volume:
  11
• Issue:
  12
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:cd8730215c088358611e0f5899fa17f17042834b5dd2e604399ec1df4bc6b5302ecf069357158f94d02a9cd09290a13a2f6a8faf4e7bcaa48edf677720156371
• Download URL:
  https://stacks.cdc.gov/view/cdc/89329/cdc_89329_DS1.pdf
• File Type:
  [PDF - 2.42 MB ]