Details:

Alternative Title:
PLoS Negl Trop Dis
Personal Author:
Barratt, Joel L. N. ; Lane, Meredith ; Talundzic, Emir ; Richins, Travis ; Robertson, Gemma ; ... More +
Barratt, Joel L. N. ; Lane, Meredith ; Talundzic, Emir ; Richins, Travis ; Robertson, Gemma ; Formenti, Fabio ; Pritt, Bobbi ; Verocai, Guilherme ; Nascimento de Souza, Joelma ; Mato Soares, Neci ; Traub, Rebecca ; Buonfrate, Dora ; Bradbury, Richard S. ; Less -
Description:
Strongyloidiasis is a neglected tropical disease caused by the human infective nematodes Strongyloides stercoralis, Strongyloides fuelleborni fuelleborni and Strongyloides fuelleborni kellyi. Previous large-scale studies exploring the genetic diversity of this important genus have focused on Southeast Asia, with a small number of isolates from the USA, Switzerland, Australia and several African countries having been genotyped. Consequently, little is known about the global distribution of geographic sub-variants of these nematodes and the genetic diversity that exists within the genus Strongyloides generally. We extracted DNA from human, dog and primate feces containing Strongyloides, collected from several countries representing all inhabited continents. Using a genotyping assay adapted for deep amplicon sequencing on the Illumina MiSeq platform, we sequenced the hyper-variable I and hyper-variable IV regions of the Strongyloides 18S rRNA gene and a fragment of the mitochondrial cytochrome c oxidase subunit 1 (cox1) gene from these specimens. We report several novel findings including unique S. stercoralis and S. fuelleborni genotypes, and the first identifications of a previously unknown S. fuelleborni infecting humans within Australia. We expand on an existing Strongyloides genotyping scheme to accommodate S. fuelleborni and these novel genotypes. In doing so, we compare our data to all 18S and cox1 sequences of S. fuelleborni and S. stercoralis available in GenBank (to our knowledge), that overlap with the sequences generated using our approach. As this analysis represents more than 1,000 sequences collected from diverse hosts and locations, representing all inhabited continents, it allows a truly global understanding of the population genetic structure of the Strongyloides species infecting humans, non-human primates, and domestic dogs.
Subject:
[+]
Animals
Cyclooxygenase 1
Dogs
Feces
Genetic Variation
Genotype
High-Throughput Nucleotide Sequencing
Humans
Neglected Diseases
Primates
RNA, Ribosomal, 18S
Sequence Analysis, DNA
Strongyloides
Strongyloides Stercoralis
Strongyloidiasis

Source:
PLoS Negl Trop Dis. 13(9) ;
Pubmed ID:
31525192
Pubmed Central ID:
PMC6762204
Document Type:
Journal Article ;
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:f5dcb0f73db57b290a2bf422e72c23c00c78bd4219fda0f013fb4c38f2d4b713
File Type:

Supporting Files

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  	pntd.0007609.s004.fasta	bin
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