Development of a workflow for identification of nuclear genotyping markers for Cyclospora cayetanensis

Houghton, Katelyn A.; Lomsadze, Alexandre; Park, Subin; Nascimento, Fernanda S.; Barratt, Joel; Arrowood, Michael J.; VanRoey, Erik; Talundzic, Eldin; Borodovsky, Mark; Qvarnstrom, Yvonne

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Development of a workflow for identification of nuclear genotyping markers for Cyclospora cayetanensis

April 10 2020
By Houghton, Katelyn A. ; Lomsadze, Alexandre ; Park, Subin ; ...
Source: Parasite. 2020; 27

[PDF-479.68 KB]

English

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Alternative Title:

Parasite
Personal Author:

Houghton, Katelyn A. ; Lomsadze, Alexandre ; Park, Subin ; Nascimento, Fernanda S. ; Barratt, Joel ; ... More +

Houghton, Katelyn A. ; Lomsadze, Alexandre ; Park, Subin ; Nascimento, Fernanda S. ; Barratt, Joel ; Arrowood, Michael J. ; VanRoey, Erik ; Talundzic, Eldin ; Borodovsky, Mark ; Qvarnstrom, Yvonne Less -
Description:

Cyclospora cayetanensis is an intestinal parasite responsible for the diarrheal illness, cyclosporiasis. Molecular genotyping, using targeted amplicon sequencing, provides a complementary tool for outbreak investigations, especially when epidemiological data are insufficient for linking cases and identifying clusters. The goal of this study was to identify candidate genotyping markers using a novel workflow for detection of segregating single nucleotide polymorphisms (SNPs) in C. cayetanensis genomes. Four whole C. cayetanensis genomes were compared using this workflow and four candidate markers were selected for evaluation of their genotyping utility by PCR and Sanger sequencing. These four markers covered 13 SNPs and resolved parasites from 57 stool specimens, differentiating C. cayetanensis into 19 new unique genotypes.
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Cyclospora Cayetanensis Cyclosporiasis Genotyping Research Article
Source:

Parasite. 2020; 27
Pubmed ID:

32275020
Pubmed Central ID:

PMC7147239
Document Type:

Journal Article
Collection(s):

CDC Public Access
Main Document Checksum:

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urn:sha256:6096af7300c5a077348566c60e4776947b022a7ff593511afb43a3cae47f1e3d
Download URL:

https://stacks.cdc.gov/view/cdc/88997/cdc_88997_DS1.pdf
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	parasite-27-24-fig1.gif	gif
	parasite-27-24-fig1.jpg	jpeg
	parasite-27-24-fig1_small.gif	gif
	parasite-27-24-fig1_small.jpg	jpeg
	parasite-27-24-s1.zip	zip
	parasite-27-24.nxml	xml

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