The limit of detection of the BioFire® FilmArray® gastrointestinal panel for the foodborne parasite Cyclospora cayetanensis

Details

• Alternative Title:
  Diagn Microbiol Infect Dis
• Personal Author:
  Peterson, Anna ; Richins, Travis ; Houghton, Katelyn ; Mishina, Margarita ; Sharma, Suresh ; Sambhara, Suryaprakash ; Jacobson, David ; Qvarnstrom, Yvonne ; Cama, Vitaliano
• Description:
  Cyclosporiasis is a foodborne diarrheal illness caused by the parasite Cyclospora cayetanensis. The BioFire® FilmArray® gastrointestinal (FilmArray GI) panel is a common method for diagnosing cyclosporiasis from clinical stool samples. The currently published limit of detection (LOD) of this panel is in genome equivalents; however, it is unclear how this relates to the number of C. cayetanensis oocysts in a clinical sample. In this study, we developed a technique to determine the LOD in terms of oocysts, using a cell sorter to sort 1 to 50 C. cayetanensis oocyst(s) previously purified from three human stool sources. We found the FilmArray GI panel detected samples with ≥20 C. cayetanensis oocysts in 100% of replicates, with varying detection among samples with 1, 5, or 10 C. cayetanensis oocysts. This method provides a parasitologically relevant LOD that should enable comparison among C. cayetanensis detection techniques, including the FilmArray GI panel.
• Subjects:
  Animals Cyclospora Cyclosporiasis Feces Humans Limit Of Detection Oocysts Parasites
• Keywords:
  BioFire FilmArray Gastrointestinal Panel Cyclospora Cayetanensis Oocyst Parasitic Disease
• Source:
  Diagn Microbiol Infect Dis. 107(2):116030
• Pubmed ID:
  37572510
• Pubmed Central ID:
  PMC10530562
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  107
• Issue:
  2
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:2fd370a22adece3b611c655d2a6b7b35e66bc51cd2963a741944ad0b1988b818f51b2f18a64cc4f8638923a1ba673a5dae1a5fad91832764a4b060584332b256
• Download URL:
  https://stacks.cdc.gov/view/cdc/133524/cdc_133524_DS1.pdf
• File Type:
  [PDF - 239.30 KB ]