The limit of detection of the BioFire® FilmArray® gastrointestinal panel for the foodborne parasite Cyclospora cayetanensis

Peterson, Anna; Richins, Travis; Houghton, Katelyn; Mishina, Margarita; Sharma, Suresh; Sambhara, Suryaprakash; Jacobson, David; Qvarnstrom, Yvonne; Cama, Vitaliano

doi:10.1016/j.diagmicrobio.2023.116030

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The limit of detection of the BioFire® FilmArray® gastrointestinal panel for the foodborne parasite Cyclospora cayetanensis

10 2023
By Peterson, Anna ; Richins, Travis ; Houghton, Katelyn ; ...
Source: Diagn Microbiol Infect Dis. 107(2):116030

Public Access Version Available on: October 01, 2024, 12:00 AM

Please check back on the date listed above.

English

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Alternative Title:

Diagn Microbiol Infect Dis
Personal Author:

Peterson, Anna ; Richins, Travis ; Houghton, Katelyn ; Mishina, Margarita ; Sharma, Suresh ; ... More +

Peterson, Anna ; Richins, Travis ; Houghton, Katelyn ; Mishina, Margarita ; Sharma, Suresh ; Sambhara, Suryaprakash ; Jacobson, David ; Qvarnstrom, Yvonne ; Cama, Vitaliano Less -
Description:

Cyclosporiasis is a foodborne diarrheal illness caused by the parasite Cyclospora cayetanensis. The BioFire® FilmArray® gastrointestinal (FilmArray GI) panel is a common method for diagnosing cyclosporiasis from clinical stool samples. The currently published limit of detection (LOD) of this panel is in genome equivalents; however, it is unclear how this relates to the number of C. cayetanensis oocysts in a clinical sample. In this study, we developed a technique to determine the LOD in terms of oocysts, using a cell sorter to sort 1 to 50 C. cayetanensis oocyst(s) previously purified from three human stool sources. We found the FilmArray GI panel detected samples with ≥20 C. cayetanensis oocysts in 100% of replicates, with varying detection among samples with 1, 5, or 10 C. cayetanensis oocysts. This method provides a parasitologically relevant LOD that should enable comparison among C. cayetanensis detection techniques, including the FilmArray GI panel.
Keywords:

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Animals BioFire FilmArray Gastrointestinal Panel Cyclospora Cyclospora Cayetanensis Cyclosporiasis Feces Humans Limit Of Detection Oocyst Oocysts Parasites Parasitic Disease
Source:

Diagn Microbiol Infect Dis. 107(2):116030
Pubmed ID:

37572510
Pubmed Central ID:

PMC10530562
Document Type:

Journal Article
Funding:

CC999999/ImCDC/Intramural CDC HHSUnited States/
Collection(s):

CDC Public Access
Main Document Checksum:

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urn:sha256:f54a72c4cc1cf47078ffc8c03ce79b0cc49ca15f03aaff4b238c44166a165649
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