Direct Sequencing of SARS-Coronavirus S and N Genes from Clinical Specimens Shows Limited Variation

Details

• Alternative Title:
  J Infect Dis
• Personal Author:
  Tong, Suxiang ; Lingappa, Jairam R. ; Chen, Qi ; Shu, Bo ; LaMonte, Ashley C. ; Cook, Byron T. ; Birge, Charryse ; Wang Chern, Shur-wern ; Liu, Xin ; Galloway, Renee ; Le Quynh, Mai ; Wai, Fu Ng ; Yang, Jyh-Yuan ; Butany, Jagdish ; Comer, James A. ; Monroe, Stephan S. ; Beard, Suzanne R. ; Ksiazek, Thomas G. ; Erdman, Dean ; Rota, Paul A. ; Pallansch, Mark A. ; Anderson, Larry J.
• Description:
  Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) emerged, in November 2002, as a novel agent causing severe respiratory illness. To study sequence variation in the SARS-CoV genome, we determined the nucleic acid sequence of the S and N genes directly from clinical specimens from 10 patients--1 specimen with no matched SARS-CoV isolate, from 2 patients; multiple specimens from 3 patients; and matched clinical-specimen/cell-culture-isolate pairs from 6 patients. We identified 3 nucleotide substitutions that were most likely due to natural variation and 2 substitutions that arose after cell-culture passage of the virus. These data demonstrate the overall stability of the S and N genes of SARS-CoV over 3 months during which a minimum of 4 generations for transmission events occurred. These findings are a part of the expanding investigation of the evolution of how this virus adapts to a new host.
• Subjects:
  Amino Acid Substitution Genome, Viral Humans Major Articles And Brief Reports Membrane Glycoproteins Mutation, Missense Nucleocapsid Proteins Point Mutation Polymorphism, Genetic RNA, Viral SARS Virus Severe Acute Respiratory Syndrome Spike Glycoprotein, Coronavirus Viral Envelope Proteins

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• Source:
  J Infect Dis. 2004; 190(6):1127-1131
• Pubmed ID:
  15319863
• Pubmed Central ID:
  PMC7199896
• Document Type:
  Journal Article
• Volume:
  190
• Issue:
  6
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:834f3d4b18304972edc0a0bf556f9f71abb825dc697f26bdc81c20ebb4da4cf9
• Download URL:
  https://stacks.cdc.gov/view/cdc/87730/cdc_87730_DS1.pdf
• File Type:
  [PDF - 83.50 KB ]