Nationwide Monitoring for Plasmodium falciparum Drug-Resistance Alleles to Chloroquine, Sulfadoxine, and Pyrimethamine, Haiti, 2016–2017

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Rogier, Eric ; Herman, Camelia ; Huber, Curtis S. ; Hamre, Karen E.S. ; Pierre, Baby ; Mace, Kimberly E. ; Présumé, Jacquelin ; Mondélus, Gina ; Romilus, Ithamare ; Elismé, Tamara ; Eisele, Thomas P. ; Druetz, Thomas ; Existe, Alexandre ; Boncy, Jacques ; Lemoine, Jean F. ; Udhayakumar, Venkatachalam ; Chang, Michelle A.
• Description:
  Haiti is striving for zero local malaria transmission by the year 2025. Chloroquine remains the first-line treatment, and sulfadoxine/pyrimethamine (SP) has been used for mass drug-administration pilot programs. In March 2016, nationwide molecular surveillance was initiated to assess molecular resistance signatures for chloroquine and SP. For 778 samples collected through December 2017, we used Sanger sequencing to investigate putative resistance markers to chloroquine (Pfcrt codons 72, 74, 75, and 76), sulfadoxine (Pfdhps codons 436, 437, 540, 581, 613), and pyrimethamine (Pfdhfr codons 50, 51, 59, 108, 164). No parasites harbored Pfcrt point mutations. Prevalence of the Pfdhfr S108N single mutation was 47%, and we found the triple mutant Pfdhfr haplotype (108N, 51I, and 59R) in a single isolate. We observed no Pfdhps variants except in 1 isolate (A437G mutation). These data confirm the lack of highly resistant chloroquine and SP alleles in Haiti and support the continued use of chloroquine and SP.
• Subjects:
  Alleles Antimalarials Chloroquine Drug Resistance Humans Malaria, Falciparum Mutation Plasmodium Falciparum Pyrimethamine Sulfadoxine
• Source:
  Emerg Infect Dis. 26(5):902-909
• Pubmed ID:
  32310062
• Pubmed Central ID:
  PMC7181918
• Document Type:
  Journal Article
• Place as Subject:
  Haiti
• Volume:
  26
• Issue:
  5
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:8eebbfd9e1934d5a107b6f063089dd21ced060b01a0adfee6e8a79beeca04f67
• Download URL:
  https://stacks.cdc.gov/view/cdc/87651/cdc_87651_DS1.pdf
• File Type:
  [PDF - 1.44 MB ]