Spike gene deletion quasispecies in serum of patient with acute MERS‐CoV infection

Details

• Alternative Title:
  J Med Virol
• Personal Author:
  Lu, Xiaoyan ; Rowe, Lori A. ; Frace, Michael ; Stevens, James ; Abedi, Glen R. ; Elnile, Osman ; Banassir, Taleb ; Al‐Masri, Malak ; Watson, John T. ; Assiri, Abdullah ; Erdman, Dean D.
• Description:
  The spike glycoprotein of the Middle East respiratory coronavirus (MERS-CoV) facilitates receptor binding and cell entry. During investigation of a multi-facility outbreak of MERS-CoV in Taif, Saudi Arabia, we identified a mixed population of wild-type and variant sequences with a large 530 nucleotide deletion in the spike gene from the serum of one patient. The out of frame deletion predicted loss of most of the S2 subunit of the spike protein leaving the S1 subunit with an intact receptor binding domain. This finding documents human infection with a novel genetic variant of MERS-CoV present as a quasispecies. J. Med. Virol. 89:542-545, 2017. © 2016 Wiley Periodicals, Inc.
• Subjects:
  Coronavirus Infections Disease Outbreaks Genetic Variation Humans Middle East Respiratory Syndrome Coronavirus Sequence Deletion Serum Spike Glycoprotein, Coronavirus
• Source:
  J Med Virol. 89(3):542-545
• Pubmed ID:
  27486688
• Pubmed Central ID:
  PMC7166981
• Document Type:
  Journal Article
• Place as Subject:
  Saudi Arabia
• Volume:
  89
• Issue:
  3
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:cbc0705578d5024358572258f2412e0332aa7ead84c785ddceff5ef99c29a8e9
• Download URL:
  https://stacks.cdc.gov/view/cdc/87087/cdc_87087_DS1.pdf
• File Type:
  [PDF - 338.39 KB ]