Laboratory Validation of the Sandfly Fever Virus Antigen Assay

Details

• Alternative Title:
  J Am Mosq Control Assoc
• Personal Author:
  Reeves, Will K. ; Szymczak, Mitchell Scott ; Burkhalter, Kristen L. ; Miller, Myrna M.
• Description:
  Sandfly fever group viruses in the genus Phlebovirus (family Bunyaviridae) are widely distributed across the globe and are a cause of disease in military troops and indigenous peoples. We assessed the laboratory sensitivity and specificity of the Sand Fly Fever Virus Antigen Assay, a rapid dipstick assay designed to detect sandfly fever Naples virus (SFNV) and Toscana virus (TOSV) against a panel of phleboviruses. The assay detected SFNV and TOSV, as well as other phleboviruses including Aguacate, Anahanga, Arumowot, Chagres, and Punta Toro viruses. It did not detect sandfly fever Sicilian, Heartland, Rio Grande, or Rift Valley fever viruses. It did not produce false positive results in the presence of uninfected sand flies (Lutzomyia longipalpis) or Cache Valley virus, a distantly related bunyavirus. Results from this laboratory evaluation suggest that this assay may be used as a rapid field-deployable assay to detect sand flies infected with TOSV and SFNV, as well as an assortment of other phleboviruses.
• Subjects:
  Animals Bunyamwera Virus Immunoassay Phlebovirus Psychodidae Sandfly Fever Naples Virus
• Keywords:
  Assay Dipstick Sandfly Fever Toscana Virus
• Source:
  J Am Mosq Control Assoc. 31(4):380-383
• Pubmed ID:
  26675463
• Pubmed Central ID:
  PMC7111559
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  31
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:a73b0a2fca3ec975f6a3e023a96dc6002c0adbe34b4290230a640865bd47e3f9
• Download URL:
  https://stacks.cdc.gov/view/cdc/86390/cdc_86390_DS1.pdf
• File Type:
  [PDF - 44.56 KB ]