EVALUATING THE USE OF COMMERCIAL WEST NILE VIRUS ANTIGENS AS POSITIVE CONTROLS IN THE RAPID ANALYTE MEASUREMENT PLATFORM WEST NILE VIRUS ASSAY

Details

• Alternative Title:
  J Am Mosq Control Assoc
• Personal Author:
  BURKHALTER, KRISTEN L. ; SAVAGE, HARRY M.
• Description:
  We evaluated the utility of 2 types of commercially available antigens as positive controls in the Rapid Analyte Measurement Platform (RAMP®) West Nile virus (WNV) assay. Purified recombinant WNV envelope antigens and whole killed virus antigens produced positive RAMP results and either type would be useful as a positive control. Killed virus antigens provide operational and economic advantages and we recommend their use over purified recombinant antigens. We also offer practical applications for RAMP positive controls and recommendations for preparing them.
• Subjects:
  Animals Antigens, Viral Culicidae Immunoassay Insect Vectors Polymerase Chain Reaction Reproducibility Of Results West Nile Fever West Nile Virus
• Keywords:
  Commercial Antigen Positive Control RAMP® Test WNV
• Source:
  J Am Mosq Control Assoc. 31(4):371-374
• Pubmed ID:
  26675461
• Pubmed Central ID:
  PMC7111257
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/
• Volume:
  31
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:2ad5e3e370311c1551ae72ff42c16f962b7017109d0fe9653be6cdb80f176566
• Download URL:
  https://stacks.cdc.gov/view/cdc/86389/cdc_86389_DS1.pdf
• File Type:
  [PDF - 55.03 KB ]