Details:

Alternative Title:
PLoS One
Personal Author:
Triana-Baltzer, Gallen B. ; Gubareva, Larisa V. ; Klimov, Alexander I. ; Wurtman, David F. ; Moss, Ronald B. ; ... More +
Triana-Baltzer, Gallen B. ; Gubareva, Larisa V. ; Klimov, Alexander I. ; Wurtman, David F. ; Moss, Ronald B. ; Hedlund, Maria ; Larson, Jeffrey L. ; Belshe, Robert B. ; Fang, Fang Less -
Description:
Antiviral drug resistance for influenza therapies remains a concern due to the high prevalence of H1N1 2009 seasonal influenza isolates which display H274Y associated oseltamivir-resistance. Furthermore, the emergence of novel H1N1 raises the potential that additional reassortments can occur, resulting in drug resistant virus. Thus, additional antiviral approaches are urgently needed. DAS181 (Fludase), a sialidase fusion protein, has been shown to have inhibitory activity against a large number of seasonal influenza strains and a highly pathogenic avian influenza (HPAI) strain (H5N1). Here, we examine the in vitro activity of DAS181 against a panel of 2009 oseltamivir-resistant seasonal H1N1 clinical isolates. The activity of DAS181 against nine 2009, two 2007, and two 2004 clinical isolates of seasonal IFV H1N1 was examined using plaque number reduction assay on MDCK cells. DAS181 strongly inhibited all tested isolates. EC50 values remained constant against isolates from 2004, 2007, and 2009, suggesting that there was no change in DAS181 sensitivity over time. As expected, all 2007 and 2009 isolates were resistant to oseltamivir, consistent with the identification of the H274Y mutation in the NA gene of all these isolates. Interestingly, several of the 2007 and 2009 isolates also exhibited reduced sensitivity to zanamivir, and accompanying HA mutations near the sialic acid binding site were observed. DAS181 inhibits IFV that is resistant to NAIs. Thus, DAS181 may offer an alternative therapeutic option for seasonal or pandemic IFVs that become resistant to currently available antiviral drugs.
Subject:
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Animals
Antiviral Agents
Binding Sites
Caco-2 Cells
Cell Proliferation
Dogs
Humans
Influenza, Human
Mutation
N-Acetylneuraminic Acid
Neuraminidase
Orthomyxoviridae
Oseltamivir
Recombinant Fusion Proteins
Research Article
Virology/Antivirals, Including Modes Of Action And Resistance
Zanamivir

Source:
PLoS One. 2009; 4(11)
Pubmed ID:
19893749
Pubmed Central ID:
PMC2770896
Document Type:
Journal Article
Funding:
U01 AI070281/AI/NIAID NIH HHS/United States ; HHSN266200600015C/PHS HHS/United States
U01 AI070281/AI/NIAID NIH HHS/United States ; HHSN266200600015C/PHS HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:87969e83244536f12e5967e060de15d234e4240fc701312df4952323dba65d25
File Type:

Supporting Files

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