Multi-Walled Carbon Nanotube-Induced Gene Expression Biomarkers for Medical and Occupational Surveillance

Snyder-Talkington, Brandi N.; Dong, Chunlin; Singh, Salvi; Raese, Rebecca; Qian, Yong; Porter, Dale W.; Wolfarth, Michael G.; Guo, Nancy L.

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Multi-Walled Carbon Nanotube-Induced Gene Expression Biomarkers for Medical and Occupational Surveillance

Supporting Files

May 29 2019
By Snyder-Talkington, Brandi N. ; Dong, Chunlin ; Singh, Salvi ; ...

File Language:

English

Details

Alternative Title:

Int J Mol Sci
Personal Author:

Snyder-Talkington, Brandi N. ; Dong, Chunlin ; Singh, Salvi ; Raese, Rebecca ; Qian, Yong ; Porter, Dale W. ; Wolfarth, Michael G. ; Guo, Nancy L.
Description:

As the demand for multi-walled carbon nanotube (MWCNT) incorporation into industrial and biomedical applications increases, so does the potential for unintentional pulmonary MWCNT exposure, particularly among workers during manufacturing. Pulmonary exposure to MWCNTs raises the potential for development of lung inflammation, fibrosis, and cancer among those exposed; however, there are currently no effective biomarkers for detecting lung fibrosis or predicting the risk of lung cancer resulting from MWCNT exposure. To uncover potential mRNAs and miRNAs that could be used as markers of exposure, this study compared in vivo mRNA and miRNA expression in lung tissue and blood of mice exposed to MWCNTs with in vitro mRNA and miRNA expression from a co-culture model of human lung epithelial and microvascular cells, a system previously shown to have a higher overall genome-scale correlation with mRNA expression in mouse lungs than either cell type grown separately. Concordant mRNAs and miRNAs identified by this study could be used to drive future studies confirming human biomarkers of MWCNT exposure. These potential biomarkers could be used to assess overall worker health and predict the occurrence of MWCNT-induced diseases.
Subjects:

Animals Article Biomarkers Cell Line Cells, Cultured Humans Lung Lung Diseases Lung Fibrosis Male Mice Mice, Inbred C57BL MicroRNAs Multi-walled Carbon Nanotubes MWCNT Nanotubes, Carbon Occupational Exposure RNA, Messenger
Source:

Int J Mol Sci. 2019; 20(11)
Pubmed ID:

31146342
Pubmed Central ID:

PMC6600433
Document Type:

Journal Article
Funding:

R01 ES021764/ES/NIEHS NIH HHS/United States ; R01ES021764/NIH HHS/National Institutes of Health/United States
Volume:

20
Issue:

11
Collection(s):

CDC Public Access
Main Document Checksum:

urn:sha256:03ba5c8dfee8cff3e1f5fbdf5a925eb0373d7f3c29d93eb1494b0342b34e56ec
Download URL:

https://stacks.cdc.gov/view/cdc/84228/cdc_84228_DS1.pdf
File Type:

[PDF - 800.10 KB ]

File Language:

English

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