Detection of Cryptosporidium Recovered from Large-Volume Water Samples Using Dead-End Ultrafiltration

Kahler, Amy M.; Hill, Vincent R.

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Detection of Cryptosporidium Recovered from Large-Volume Water Samples Using Dead-End Ultrafiltration

Supporting Files

2020
By Kahler, Amy M. ; Hill, Vincent R.

File Language:

English

Details

Alternative Title:

Methods Mol Biol
Personal Author:

Kahler, Amy M. ; Hill, Vincent R.
Description:

The procedure described here provides instructions for detection of Cryptosporidium recovered from large-volume water samples. Water samples are collected by dead-end ultrafiltration in the field and ultrafilters are processed in a laboratory. Microbes recovered from the filters are further concentrated and subjected to Cryptosporidium isolation or nucleic acid extraction methods for the detection of Cryptosporidium oocysts or Cryptosporidium DNA.
Subjects:

Article Cryptosporidium Dead-end Ultrafiltration IMS Microscopy Real-time PCR Water
Source:

Methods Mol Biol. 2052:23-41
Pubmed ID:

31452155
Pubmed Central ID:

PMC6953165
Document Type:

Journal Article
Funding:

CC999999/ImCDC/Intramural CDC HHS/United States
Volume:

2052
Collection(s):

CDC Public Access
Main Document Checksum:

urn:sha256:4b66640b1e0a61bd23fe278e549f582adcd76fb4eb7243d4ac2d17397e9ca04f
Download URL:

https://stacks.cdc.gov/view/cdc/84123/cdc_84123_DS1.pdf
File Type:

[PDF - 675.69 KB ]

File Language:

English

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