Details:

Alternative Title:
J Anal Toxicol
Personal Author:
Kalb, Suzanne R. ; Baudys, Jakub ; Kiernan, Kaitlyn ; Wang, Dongxia ; Becher, François ; ... More +
Kalb, Suzanne R. ; Baudys, Jakub ; Kiernan, Kaitlyn ; Wang, Dongxia ; Becher, François ; Barr, John R. Less -
Description:
Botulinum neurotoxins (BoNTs) are a family of protein toxins consisting of seven known serotypes (BoNT/A-BoNT/G) and multiple subtypes within the serotypes, and all of which cause the disease botulism-a disease of great public health concern. Accurate detection of BoNTs in human clinical samples is therefore an important public health goal. To achieve this goal, our laboratory developed a mass spectrometry-based assay detecting the presence of BoNT via its enzymatic activity on a peptide substrate. Recently, publications reported the use of new peptide substrates to detect BoNT/A and /B with improved results over other peptide substrates. However, the authors did not provide results of their peptide substrate on multiple subtypes of BoNT. In this work, we describe the results of testing the new substrates with multiple BoNT/A and /B subtypes and find that the substrates cannot detect many subtypes of BoNT/A and /B.
Subjects:
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Bological Assay Botulinum Toxins, Type A Botulinum Toxins/analysis Botulism Enkephalins Humans Limit Of Detection Mass Spectrometry Peptides Protein Precursors
Source:
J Anal Toxicol. 44(2):173-179
Pubmed ID:
31287544
Pubmed Central ID:
PMC6949424
Document Type:
Journal Article
Funding:
CC999999/ImCDC/Intramural CDC HHS/United States
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:a64730b7965a20a8d9e3cdf3403b1ac17597ccf79fa6e0d55cbb1cf0e56cc2c8
Download URL:
https://stacks.cdc.gov/view/cdc/84115/cdc_84115_DS1.pdf
File Type:

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