Early Diagnosis of Tularemia by Flow Cytometry, Czech Republic, 2003–20151

Chrdle, Aleš; Tinavská, Pavlína; Dvořáčková, Olga; Filipová, Pavlína; Hnetilová, Věra; Žampach, Pavel; Batistová, Květoslava; Chmelík, Václav; Semper, Amanda E.; Beeching, Nick J.

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Early Diagnosis of Tularemia by Flow Cytometry, Czech Republic, 2003–20151

Supporting Files Public Domain

October 2019
By Chrdle, Aleš ; Tinavská, Pavlína ; Dvořáčková, Olga ; ...

File Language:

English

Details

Alternative Title:

Emerg Infect Dis
Personal Author:

Chrdle, Aleš ; Tinavská, Pavlína ; Dvořáčková, Olga ; Filipová, Pavlína ; Hnetilová, Věra ; Žampach, Pavel ; Batistová, Květoslava ; Chmelík, Václav ; Semper, Amanda E. ; Beeching, Nick J.
Description:

We retrospectively assessed the utility of a flow cytometry-based test quantifying the percentage of CD3+ T cells with the CD4-/CD8- phenotype for predicting tularemia diagnoses in 64 probable and confirmed tularemia patients treated during 2003-2015 and 342 controls with tularemia-like illnesses treated during 2012-2015 in the Czech Republic. The median percentage of CD3+/CD4-/CD8- T cells in peripheral blood was higher in tularemia patients (19%, 95% CI 17%-22%) than in controls (3%, 95% CI 2%-3%). When we used 8% as the cutoff, this test's sensitivity was 0.953 and specificity 0.895 for distinguishing cases from controls. The CD3+/CD4-/CD8- T cells increased a median of 7 days before tularemia serologic test results became positive. This test supports early presumptive diagnosis of tularemia for clinically suspected cases 7-14 days before diagnosis can be confirmed by serologic testing in regions with low prevalences of tularemia-like illnesses.
Subjects:

Bacteria Czech Republic Diagnosis Double-negative T Cells Early Diagnosis Early Diagnosis Of Tularemia By Flow Cytometry, Czech Republic, 2003–2015 Flow Cytometry Francisella Tularensis Gamma Delta T Cells Intracellular Pathogens Peripheral Blood Research ROC Curve T Cells Tularemia Work-up γδ T Cells
Source:

Emerg Infect Dis. 25(10):1919-1927
Pubmed ID:

31538915
Pubmed Central ID:

PMC6759275
Document Type:

Journal Article
Volume:

25
Issue:

10
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:d5f02597cb7699b0abc106eaec13a257f8b1d7b45051bc4ea799a8f856788782
Download URL:

https://stacks.cdc.gov/view/cdc/82945/cdc_82945_DS1.pdf
File Type:

[PDF - 1.22 MB ]

File Language:

English

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