In Vitro Toxicity Assessment of Emitted Materials Collected during the Manufacture of Water Pipe Plastic Linings

Details

• Alternative Title:
  Inhal Toxicol
• Personal Author:
  Kobos, Lisa ; Teimouri Sendesi, Seyedeh Mahboobeh ; Whelton, Andrew J. ; Boor, Brandon E ; Howarter, John A ; Shannahan, Jonathan
• Description:
  US water infrastructure is in need of widespread repair due to age-related deterioration. Currently, the cured-in-place (CIPP) procedure is the most common method for water pipe repair. This method involves the on-site manufacture of a new polymer composite plastic liner within the damaged pipe. The CIPP process can release materials resulting in occupational and public health concerns. To understand hazards associated with CIPP-related emission exposures, an | toxicity assessment was performed. | Mouse alveolar epithelial and alveolar macrophage cell lines and condensates collected at 3 worksites utilizing styrene-based resins were utilized for evaluations. All condensate samples were normalized based on the major emission component, styrene. Further, a styrene-only exposure group was used as a control to determine mixture related toxicity. | Cytotoxicity differences were observed between worksite samples, with the CIPP worksite 4 sample inducing the most cell death. A proteomic evaluation was performed, which demonstrated styrene-, worksite-, and cell-specific alterations. This examination of protein expression changes determined potential biomarkers of exposure including transglutaminase 2, advillin, collagen type 1, perilipin-2, and others. Pathway analysis of exposure-induced proteomic alterations identified MYC and p53 to be regulators of cellular responses. Protein changes were also related to pathways involved in cell damage, immune response, and cancer. | Together these findings demonstrate potential risks associated with the CIPP procedure as well as variations between worksites regarding emissions and toxicity. Our evaluation identified biological pathways that require a future evaluation and also demonstrates that exposure assessment of CIPP worksites should examine multiple chemical components beyond styrene, as many cellular responses were styrene-independent.
• Subjects:
  Air Pollutants, Occupational Animals Article Cancer Cell Survival Cured-In-Place Pipe Dose-Response Relationship, Drug Environmental Monitoring Epithelial Cells Gene Expression Macrophages Macrophages, Alveolar Manufactured Materials Mice Oxidative Stress Proteome Proteomics RAW 264.7 Cells Styrene Workplace

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• Source:
  Inhal Toxicol. 31(4):131-146
• Pubmed ID:
  31187656
• Pubmed Central ID:
  PMC6639800
• Document Type:
  Journal Article
• Funding:
  R00 ES024392/ES/NIEHS NIH HHS/United States ; T42 OH008672/OH/NIOSH CDC HHS/United States ; T42OH008672/ACL HHS/United States
• Volume:
  31
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:d0f38ab468e11bda4f9b6800f37be461c557b88025ad829f7c0a74eab3070be5
• Download URL:
  https://stacks.cdc.gov/view/cdc/81922/cdc_81922_DS1.pdf
• File Type:
  [PDF - 1.64 MB ]