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Development and optimization of a non-enzymatic method of leukocyte isolation from macaque tissues
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10 2014
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Source: J Med Primatol. 43(5):360-363
[PDF-670.96 KB]
Details:
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Alternative Title:J Med Primatol
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Personal Author:
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Description:Background and Methods
Cell isolation from macaque tissues involves laborious enzymatic digestion. The Medimachine provides a simpler, quicker non-enzymatic method, yielding 1.5–5 million cells/g of vaginal or rectal tissue from pigtailed macaques.
Results and Conclusions
Flow cytometry analysis of the two methods revealed similar levels of cell viability and most major cell lineage and activation markers.
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Pubmed ID:25379593
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Pubmed Central ID:PMC6711605
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Volume:43
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Issue:5
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