Development and optimization of a non-enzymatic method of leukocyte isolation from macaque tissues

Details

• Alternative Title:
  J Med Primatol
• Personal Author:
  Pereira, Lara E. ; Makarova, Natalia ; Dobard, Charles ; Aubert, Rachael D. ; Srinivasan, Priya ; McNicholl, Janet ; Smith, James M.
• Description:
  Background and Methods
  
  Cell isolation from macaque tissues involves laborious enzymatic digestion. The Medimachine provides a simpler, quicker non-enzymatic method, yielding 1.5–5 million cells/g of vaginal or rectal tissue from pigtailed macaques.
  
  Results and Conclusions
  
  Flow cytometry analysis of the two methods revealed similar levels of cell viability and most major cell lineage and activation markers.
  
  
• Subjects:
  Animals Article Cell Separation Cell Survival Collagenase Digestion Female Flow Cytometry Leukocytes Macaca Nemestrina Medimachine Rectum Vagina

  More +
• Source:
  J Med Primatol. 43(5):360-363
• Pubmed ID:
  25379593
• Pubmed Central ID:
  PMC6711605
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHSUnited States/
• Volume:
  43
• Issue:
  5
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:0c2cbf2b40d0ee0e9be12c7b2783d24636269f330567514c103a81476abcf14b
• Download URL:
  https://stacks.cdc.gov/view/cdc/81057/cdc_81057_DS1.pdf
• File Type:
  [PDF - 670.96 KB ]