Details:

Alternative Title:
J Med Primatol
Personal Author:
Pereira, Lara E. ; Makarova, Natalia ; Dobard, Charles ; Aubert, Rachael D. ; Srinivasan, Priya ; ... More +
Pereira, Lara E. ; Makarova, Natalia ; Dobard, Charles ; Aubert, Rachael D. ; Srinivasan, Priya ; McNicholl, Janet ; Smith, James M. Less -
Description:
Background and Methods

Cell isolation from macaque tissues involves laborious enzymatic digestion. The Medimachine provides a simpler, quicker non-enzymatic method, yielding 1.5–5 million cells/g of vaginal or rectal tissue from pigtailed macaques.

Results and Conclusions

Flow cytometry analysis of the two methods revealed similar levels of cell viability and most major cell lineage and activation markers.


Subject:
[+]
Animals
Article
Cell Separation
Cell Survival
Collagenase Digestion
Female
Flow Cytometry
Leukocytes
Macaca Nemestrina
Medimachine
Rectum
Vagina
Flow Cytometry

Source:
J Med Primatol. 43(5):360-363
Pubmed ID:
25379593
Pubmed Central ID:
PMC6711605
Document Type:
Journal Article
Funding:
CC999999/Intramural CDC HHSUnited States/
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:0c2cbf2b40d0ee0e9be12c7b2783d24636269f330567514c103a81476abcf14b
File Type:

Supporting Files

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