Development and optimization of a non-enzymatic method of leukocyte isolation from macaque tissues

Details

• Alternative Title:
  J Med Primatol
• Personal Author:
  Pereira, Lara E. ; Makarova, Natalia ; Dobard, Charles ; Aubert, Rachael D. ; Srinivasan, Priya ; McNicholl, Janet ; Smith, James M.
• Description:
  Background and Methods ; Cell isolation from macaque tissues involves laborious enzymatic digestion. The Medimachine provides a simpler, quicker non-enzymatic method, yielding 1.5–5 million cells/g of vaginal or rectal tissue from pigtailed macaques. ; Results and Conclusions ; Flow cytometry analysis of the two methods revealed similar levels of cell viability and most major cell lineage and activation markers.
• Subjects:
  • Article
  • collagenase digestion
  • flow cytometry
  • Macaca nemestrina
  • Medimachine
  • Animals
  • Cell Separation
  • Cell Survival
  • Female
  • Flow Cytometry
  More +
• Source:
  J Med Primatol. 43(5):360-363
• Pubmed ID:
  25379593
• Pubmed Central ID:
  PMC6711605
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHSUnited States/
• Volume:
  43
• Issue:
  5
• Download URL:
  https://stacks.cdc.gov/view/cdc/81057/cdc_81057_DS1.pdf
• File Type:
  [PDF - 670.96 KB]
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:0c2cbf2b40d0ee0e9be12c7b2783d24636269f330567514c103a81476abcf14b