Development and optimization of a non-enzymatic method of leukocyte isolation from macaque tissues

Pereira, Lara E.; Makarova, Natalia; Dobard, Charles; Aubert, Rachael D.; Srinivasan, Priya; McNicholl, Janet; Smith, James M.

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Development and optimization of a non-enzymatic method of leukocyte isolation from macaque tissues

10 2014
By Pereira, Lara E. ; Makarova, Natalia ; Dobard, Charles ; ...
Source: J Med Primatol. 43(5):360-363

[PDF-670.96 KB]

English

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Details:

Alternative Title:

J Med Primatol
Personal Author:

Pereira, Lara E. ; Makarova, Natalia ; Dobard, Charles ; Aubert, Rachael D. ; Srinivasan, Priya ; ... More +

Pereira, Lara E. ; Makarova, Natalia ; Dobard, Charles ; Aubert, Rachael D. ; Srinivasan, Priya ; McNicholl, Janet ; Smith, James M. Less -
Description:

Background and Methods

Cell isolation from macaque tissues involves laborious enzymatic digestion. The Medimachine provides a simpler, quicker non-enzymatic method, yielding 1.5–5 million cells/g of vaginal or rectal tissue from pigtailed macaques.

Results and Conclusions

Flow cytometry analysis of the two methods revealed similar levels of cell viability and most major cell lineage and activation markers.
Subjects:

[+]

Animals Article Cell Separation Cell Survival Collagenase Digestion Female Flow Cytometry Leukocytes Macaca Nemestrina Medimachine Rectum Vagina
Source:

J Med Primatol. 43(5):360-363
Pubmed ID:

25379593
Pubmed Central ID:

PMC6711605
Document Type:

Journal Article
Funding:

CC999999/Intramural CDC HHSUnited States/
Volume:

43
Issue:

5
Collection(s):

CDC Public Access
Main Document Checksum:

[+]

urn:sha256:0c2cbf2b40d0ee0e9be12c7b2783d24636269f330567514c103a81476abcf14b
Download URL:

https://stacks.cdc.gov/view/cdc/81057/cdc_81057_DS1.pdf
File Type:

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