CDC STACKS serves as an archival repository of CDC-published products including scientific findings, journal articles, guidelines, recommendations, or other public health information authored or co-authored by CDC or funded partners.
As a repository, CDC STACKS retains documents in their original published format to ensure public access to scientific information.
i
Electron cryo-tomography of vestibular hair-cell stereocilia
-
February 26 2019
-
-
Source: J Struct Biol. 206(2):149-155
[PDF-2.80 MB]
Details:
-
Alternative Title:J Struct Biol
-
Personal Author:
-
Description:High-resolution imaging of hair-cell stereocilia of the inner ear has contributed substantially to our understanding of auditory and vestibular function. To provide three-dimensional views of the structure of stereocilia cytoskeleton and membranes, we developed a method for rapidly freezing unfixed stereocilia on electron microscopy grids, which allowed subsequent 3D imaging by electron cryo-tomography. Structures of stereocilia tips, shafts, and tapers were revealed, demonstrating that the actin paracrystal was not perfectly ordered. This sample-preparation and imaging procedure will allow for examination of structural features of stereocilia in a near-native state.
-
Subjects:
-
Source:
-
Pubmed ID:30822456
-
Pubmed Central ID:PMC6684322
-
Document Type:
-
Funding:R01 GM115972/NIGMS NIH HHS/National Institute of General Medical Sciences/United States ; P01 GM121203/NIGMS NIH HHS/National Institute of General Medical Sciences/United States ; P01 GM098412/NIGMS NIH HHS/National Institute of General Medical Sciences/United States ; P01 GM051487/NIGMS NIH HHS/National Institute of General Medical Sciences/United States ; P30 DC005983/NIDCD NIH HHS/National Institute on Deafness and Other Communication Disorders/United States ; ... More +
-
Volume:206
-
Issue:2
-
Collection(s):
-
Main Document Checksum:
-
Download URL:
-
File Type:
