Electron cryo-tomography of vestibular hair-cell stereocilia

Details

• Alternative Title:
  J Struct Biol
• Personal Author:
  Metlagel, Zoltan ; Krey, Jocelyn F. ; Song, Junha ; Swift, Mark F. ; Tivol, William J. ; Dumont, Rachel A. ; Thai, Jasmine ; Chang, Alex ; Seifikar, Helia ; Volkmann, Niels ; Hanein, Dorit ; Barr-Gillespie, Peter G. ; Auer, Manfred
• Description:
  High-resolution imaging of hair-cell stereocilia of the inner ear has contributed substantially to our understanding of auditory and vestibular function. To provide three-dimensional views of the structure of stereocilia cytoskeleton and membranes, we developed a method for rapidly freezing unfixed stereocilia on electron microscopy grids, which allowed subsequent 3D imaging by electron cryo-tomography. Structures of stereocilia tips, shafts, and tapers were revealed, demonstrating that the actin paracrystal was not perfectly ordered. This sample-preparation and imaging procedure will allow for examination of structural features of stereocilia in a near-native state.
• Subjects:
  Actin Article Cryo-electron Microscopy Hair Cells Stereocilia
• Source:
  J Struct Biol. 206(2):149-155
• Pubmed ID:
  30822456
• Pubmed Central ID:
  PMC6684322
• Document Type:
  Journal Article
• Funding:
  R01 GM115972/NIGMS NIH HHS/National Institute of General Medical Sciences/United States ; P01 GM121203/NIGMS NIH HHS/National Institute of General Medical Sciences/United States ; P01 GM098412/NIGMS NIH HHS/National Institute of General Medical Sciences/United States ; P01 GM051487/NIGMS NIH HHS/National Institute of General Medical Sciences/United States ; P30 DC005983/NIDCD NIH HHS/National Institute on Deafness and Other Communication Disorders/United States ; R01 DC002368/NIDCD NIH HHS/National Institute on Deafness and Other Communication Disorders/United States ; R01 DC011034/NIDCD NIH HHS/National Institute on Deafness and Other Communication Disorders/United States ; S10 OD012372/ODCDC CDC HHS/Office of the Director/United States
• Volume:
  206
• Issue:
  2
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:628af466874734895579a9d962e0d7c18e2eb63ec0fe05da47e6d9f2c0c5d133
• Download URL:
  https://stacks.cdc.gov/view/cdc/80523/cdc_80523_DS1.pdf
• File Type:
  [PDF - 2.80 MB ]