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Electron cryo-tomography of vestibular hair-cell stereocilia
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February 26 2019
Source: J Struct Biol. 206(2):149-155 -
Alternative Title:J Struct Biol
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Description:High-resolution imaging of hair-cell stereocilia of the inner ear has contributed substantially to our understanding of auditory and vestibular function. To provide three-dimensional views of the structure of stereocilia cytoskeleton and membranes, we developed a method for rapidly freezing unfixed stereocilia on electron microscopy grids, which allowed subsequent 3D imaging by electron cryo-tomography. Structures of stereocilia tips, shafts, and tapers were revealed, demonstrating that the actin paracrystal was not perfectly ordered. This sample-preparation and imaging procedure will allow for examination of structural features of stereocilia in a near-native state.
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Pubmed ID:30822456
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Pubmed Central ID:PMC6684322
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Funding:R01 GM115972/NIGMS NIH HHS/National Institute of General Medical Sciences/United States ; P01 GM121203/NIGMS NIH HHS/National Institute of General Medical Sciences/United States ; P01 GM098412/NIGMS NIH HHS/National Institute of General Medical Sciences/United States ; P01 GM051487/NIGMS NIH HHS/National Institute of General Medical Sciences/United States ; P30 DC005983/NIDCD NIH HHS/National Institute on Deafness and Other Communication Disorders/United States ; ... More +
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