Weaned pigs experimentally infected with Salmonella display sexually dimorphic innate immune responses without affecting pathogen colonization patterns1,2

Details

• Alternative Title:
  Transl Anim Sci
• Personal Author:
  Burdick Sanchez, Nicole C. ; Broadway, Paul R. ; Carroll, Jeffery A. ; Gart, Elena V. ; Bryan, Laura K. ; Lawhon, Sara D.
• Description:
  Sexually dimorphic innate immune responses have been observed in several species, but have not been studied in response to a live pathogen challenge in pigs. This study aimed to elucidate sexually dimorphic innate immune responses along with | translocation patterns in newly weaned pigs orally inoculated with |. Newly weaned pigs (| = 8 gilts and 12 barrows; 6.2 ± 0.2 kg BW) were obtained from a commercial swine facility and were maintained in an environmentally-controlled facility in individual pens equipped with feeders and nipple waterers. Pigs were allowed ad libitum access to a commercial non-medicated starter ration and water throughout the study. On d 12 post-weaning, pigs were anesthetized to allow placement of a temperature measuring device in the abdominal cavity for measurement of intraperitoneal temperature (TEMP). On d 17, pigs were anesthetized and fitted with indwelling jugular vein catheters. On the following day (d 18), pigs were orally inoculated with 4.7×10|. Blood samples were collected at 0.5-h intervals from -2 to 8 h, and at 8-h intervals from 8 to 72 h post-challenge. Whole blood was analyzed for complete blood cell counts. Serum was isolated for measurement of cortisol. Following collection of the 72 h sample, pigs were humanely euthanized and tissues were collected for | isolation. There was a sex × time interaction (| < 0.001) for TEMP such that gilts had a greater TEMP response to the | challenge compared to barrows. There was also a sex × time interaction (| = 0.03) for serum cortisol with gilts having decreased cortisol at 16 h yet greater cortisol at 32 h than barrows. Barrows had greater total white blood cells (17.8 vs. 16.2 ± 0.4 10| cells/μL; | < 0.01; respectively) and neutrophils (7.8 vs. 6.1 ± 0.4 10| cells/μL; | < 0.01; respectively) than gilts. However, gilts had greater lymphocytes (9.6 vs. 9.0 ± 0.2 10| cells/μL; | = 0.05; respectively) than barrows. While immune parameters were influenced by sex, there was no effect of sex (| > 0.05) on | concentrations from fecal shedding 3 d post-inoculation in the cecum, mesenteric and subiliac lymph nodes, liver, spleen, gallbladder, or kidney tissues. These data demonstrate that weaned gilts appear to produce a stronger acute phase response to a | challenge compared to barrows, without affecting the tissue translocation or shedding of |.
• Subjects:
  Article Cortisol Innate Immunity Pigs Salmonella Sexual Dimorphism
• Source:
  Transl Anim Sci. 1(1):69-76
• Pubmed ID:
  31372597
• Pubmed Central ID:
  PMC6675027
• Document Type:
  Journal Article
• Funding:
  T32 OD011083/ODCDC CDC HHS/Office of the Director/United States
• Volume:
  1
• Issue:
  1
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:1632cf44a11afb21234fdca0a894db6306c8632372354bbfb2e0c9b47f1e442b
• Download URL:
  https://stacks.cdc.gov/view/cdc/80497/cdc_80497_DS1.pdf
• File Type:
  [PDF - 1021.61 KB ]