Overview of CRISPR–Cas9 Biology

Ratner, Hannah K.; Sampson, Timothy R.; Weiss, David S.

doi:10.1101/pdb.top088849

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Overview of CRISPR–Cas9 Biology

Published Date:

December 01 2016

Publisher's site:

http://dx.doi.org/10.1101/pdb.top088849

Source:

Cold Spring Harb Protoc. 2016(12):pdb.top088849

Language:

English

[PDF-480.54 KB]

Details:

Alternative Title:

Cold Spring Harb Protoc

Personal Author:

Ratner, Hannah K. ; Sampson, Timothy R. ; Weiss, David S. ;

Description:

Prokaryotes use diverse strategies to improve fitness in the face of different environmental threats and stresses, including those posed by mobile genetic elements (e.g., bacteriophages and plasmids). To defend against these elements, many bacteria and archaea use elegant, RNA-directed, nucleic acid-targeting adaptive restriction machineries called CRISPR -: Cas (CRISPR-associated) systems. While providing an effective defense against foreign genetic elements, these systems have also been observed to play critical roles in regulating bacterial physiology during environmental stress. Increasingly, CRISPR-Cas systems, in particular the Type II systems containing the Cas9 endonuclease, have been exploited for their ability to bind desired nucleic acid sequences, as well as direct sequence-specific cleavage of their targets. Cas9-mediated genome engineering is transcending biological research as a versatile and portable platform for manipulating genetic content in myriad systems. Here, we present a systematic overview of CRISPR-Cas history and biology, highlighting the revolutionary tools derived from these systems, which greatly expand the molecular biologists' toolkit.

Subject:

[+]

Pubmed ID:

27934695

Pubmed Central ID:

PMC5738243

Document Type:

Journal Article

Funding:

P51 OD011132/ODCDC CDC HHS/Office of the Director/United States ; R01 AI110701/NIAID NIH HHS/National Institute of Allergy and Infectious Diseases Extramural Activities/United States ;

Collection(s):

CDC Public Access

Main Document Checksum:

[+]

Supporting Files:

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