Welcome to CDC Stacks | Development and Applications of CRISPR-Cas9 for Genome Engineering - 30309 | CDC Public Access
Stacks Logo
Advanced Search
Select up to three search categories and corresponding keywords using the fields to the right. Refer to the Help section for more detailed instructions.
 
 
Help
Clear All Simple Search
Advanced Search
Development and Applications of CRISPR-Cas9 for Genome Engineering
Filetype[PDF - 867.35 KB]


Details:
  • Personal Authors:
  • Keywords:
  • Pubmed ID:
    24906146
  • Pubmed Central ID:
    PMC4343198
  • Description:
    Recent advances in genome engineering technologies based on the CRISPR-associated RNA-guided endonuclease Cas9 are enabling the systematic interrogation of mammalian genome function. Analogous to the search function in modern word processors, Cas9 can be guided to specific locations within complex genomes by a short RNA search string. Using this system, DNA sequences within the endogenous genome and their functional outputs are now easily edited or modulated in virtually any organism of choice. Cas9-mediated genetic perturbation is simple and scalable, empowering researchers to elucidate the functional organization of the genome at the systems level and establish causal linkages between genetic variations and biological phenotypes. In this Review, we describe the development and applications of Cas9 for a variety of research or translational applications while highlighting challenges as well as future directions. Derived from a remarkable microbial defense system, Cas9 is driving innovative applications from basic biology to biotechnology and medicine.

  • Document Type:
    Journal Article
  • Collection(s):
    CDC Public Access
  • Funding:
    DP1 MH100706/MH/NIMH NIH HHS/United States
    DP1-MH100706/DP/NCCDPHP CDC HHS/United States
    R01 DK097768/DK/NIDDK NIH HHS/United States
    R01 NS073124/NS/NINDS NIH HHS/United States
    R01-NS 07312401),/NS/NINDS NIH HHS/United States
No Related Documents.
You May Also Like:
  1. Genome engineering using the CRISPR-Cas9 system
    Personal Author:
    Ran, F Ann; Hsu, Patrick D; Wright, Jason; Agarwala, Vineeta; Scott, David A; Zhang, Feng;
    Published:
    Oct 24 2013
    Source:
    Nat Protoc. 2013; 8(11):2281-2308.
    Description:
    Targeted nucleases are powerful tools for mediating genome alteration with high precision. The RNA-guided Cas9 nuclease from the microbial clustered regularly interspaced short palindromic repeats (CRISPR) adaptive immune system can be used to facili...
    File Type:
    Filetype[PDF - 1.94 MB]
  2. CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling
    Personal Author:
    Platt, Randall J.; Chen, Sidi; Zhou, Yang; Yim, Michael J.; Swiech, Lukasz; Kempton, Hannah R.; Dahlman, James E.; Parnas, Oren; Eisenhaure, Thomas M.; Jovanovic, Marko; Graham, Daniel B.; Jhunjhunwala, Siddharth; Xavier, Ramnik J.; Langer, Robert; Anderson, Daniel G.; Hacohen, Nir; Regev, Aviv; Feng, Guoping; Sharp, Phillip A.; Zhang, Feng;
    Published:
    Sep 25 2014
    Source:
    Cell. 2014; 159(2):440-455.
    Description:
    CRISPR-Cas9 is a versatile genome editing technology for studying the functions of genetic elements. To broadly enable the application of Cas9 in vivo, we established a Cre-dependent Cas9 knockin mouse. We demonstrated in vivo as well as ex vivo geno...
    File Type:
    Filetype[PDF - 3.28 MB]
  3. Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity
    Personal Author:
    Ran, F. Ann; Hsu, Patrick D.; Lin, Chie-Yu; Gootenberg, Jonathan S.; Konermann, Silvana; Trevino, Alexandro; Scott, David A.; Inoue, Azusa; Matoba, Shogo; Zhang, Yi; Zhang, Feng;
    Published:
    Aug 29 2013
    Source:
    Cell. 2013; 154(6):1380-1389.
    Description:
    Targeted genome editing technologies have enabled a broad range of research and medical applications. The Cas9 nuclease from the microbial CRISPR-Cas system is targeted to specific genomic loci by a 20 nt guide sequence, which can tolerate certain mi...
    File Type:
    Filetype[PDF - 1.36 MB]
  4. Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells
    Personal Author:
    Wu, Xuebing; Scott, David A.; Kriz, Andrea J.; Chiu, Anthony C.; Hsu, Patrick D.; Dadon, Daniel B.; Cheng, Albert W.; Trevino, Alexandro E.; Konermann, Silvana; Chen, Sidi; Jaenisch, Rudolf; Zhang, Feng; Sharp, Phillip A.;
    Published:
    Apr 20 2014
    Source:
    Nat Biotechnol. 32(7):670-676.
    Description:
    Bacterial type II CRISPR-Cas9 systems have been widely adapted for RNA-guided genome editing and transcription regulation in eukaryotic cells, yet their in vivo target specificity is poorly understood. Here we mapped genome-wide binding sites of a ca...
    File Type:
    Filetype[PDF - 1.31 MB]
  5. Structure and Engineering of Francisella novicida Cas9
    Personal Author:
    Hirano, Hisato; Gootenberg, Jonathan S.; Horii, Takuro; Abudayyeh, Omar O.; Kimura, Mika; Hsu, Patrick D.; Nakane, Takanori; Ishitani, Ryuichiro; Hatada, Izuho; Zhang, Feng; Nishimasu, Hiroshi; Nureki, Osamu;
    Published:
    Feb 11 2016
    Source:
    Cell. 164(5):950-961.
    Description:
    The RNA-guided endonuclease Cas9 cleaves double-stranded DNA targets complementary to the guide RNA and has been applied to programmable genome editing. Cas9-mediated cleavage requires a protospacer adjacent motif (PAM) juxtaposed with the DNA target...
    File Type:
    Filetype[PDF - 3.62 MB]