Development and Applications of CRISPR-Cas9 for Genome Engineering

Hsu, Patrick D.; Lander, Eric S.; Zhang, Feng

doi:10.1016/j.cell.2014.05.010

i

Development and Applications of CRISPR-Cas9 for Genome Engineering

Supporting Files

6 5 2014 ; 6-5-
By Hsu, Patrick D. ; Lander, Eric S. ; Zhang, Feng
http://dx.doi.org/10.1016/j.cell.2014.05.010
Available in CDC Stacks on 2015-02-27T00:00:00Z

File Language:

English

Details

Alternative Title:

Cell
Personal Author:

Hsu, Patrick D. ; Lander, Eric S. ; Zhang, Feng
Description:

Recent advances in genome engineering technologies based on the CRISPR-associated RNA-guided endonuclease Cas9 are enabling the systematic interrogation of mammalian genome function. Analogous to the search function in modern word processors, Cas9 can be guided to specific locations within complex genomes by a short RNA search string. Using this system, DNA sequences within the endogenous genome and their functional outputs are now easily edited or modulated in virtually any organism of choice. Cas9-mediated genetic perturbation is simple and scalable, empowering researchers to elucidate the functional organization of the genome at the systems level and establish causal linkages between genetic variations and biological phenotypes. In this Review, we describe the development and applications of Cas9 for a variety of research or translational applications while highlighting challenges as well as future directions. Derived from a remarkable microbial defense system, Cas9 is driving innovative applications from basic biology to biotechnology and medicine.
Subjects:
Source:

Cell. 2014; 157(6):1262-1278
Pubmed ID:

24906146
Pubmed Central ID:

PMC4343198
Document Type:

Journal Article
Funding:

DP1-MH100706/DP/NCCDPHP CDC HHSUnited States/ ; R01-NS 07312401),/NS/NINDS NIH HHSUnited States/ ; DP1 MH100706/MH/NIMH NIH HHSUnited States/ ; R01 NS073124/NS/NINDS NIH HHSUnited States/ ; R01 DK097768/DK/NIDDK NIH HHSUnited States/
Volume:

157
Issue:

6
Download URL:

https://stacks.cdc.gov/view/cdc/30309/cdc_30309_DS1.pdf
File Type:

[PDF - 867.35 KB]
Collection(s):

CDC Public Access
Main Document Checksum:

urn:sha256:1e08cdc763193a5e69c1e641da08a3c8374be670192868efa755200e1c2f6ac3

File Language:

English

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