Natural Vertical Transmission of Zika Virus in Larval Aedes aegypti Populations, Morelos, Mexico

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Izquierdo-Suzán, Mónica ; Zárate, Selene ; Torres-Flores, Jesús ; Correa-Morales, Fabián ; González-Acosta, Cassandra ; Sevilla-Reyes, Edgar E. ; Lira, Rosalia ; Alcaraz-Estrada, Sofía L. ; Yocupicio-Monroy, Martha
• Description:
  We characterized natural vertical transmission of Zika virus in pools of Aedes aegypti larvae hatched from eggs collected in Jojutla, Morelos, Mexico. Of the 151 pools analyzed, 17 tested positive for Zika virus RNA; infectious Zika virus was successfully isolated from 1 of the larvae pools (31N) in C6/36 cells. Real-time quantitative PCR and indirect immunofluorescence assays confirmed the identity of the isolate, named Zika virus isolate 31N; plaque assays in Vero cells demonstrated the isolate's infectivity in a mammalian cell line. We obtained the complete genome of Zika virus isolate 31N by next-generation sequencing and identified 3 single-nucleotide variants specific to Zika virus isolate 31N using the meta-CATS tool. These results demonstrate the occurrence of natural vertical transmission of Zika virus in wild Ae. aegypti mosquitoes and suggest that this transmission mode could aid in the spread and maintenance of Zika virus in nature.
• Subjects:
  Aedes Aegypti Larvae Mosquitoes Natural Vertical Transmission Research Vector-borne Infections Viruses Zika Virus
• Source:
  Emerg Infect Dis. 25(8):1477-1484
• Document Type:
  Journal Article
• Place as Subject:
  Mexico
• Location:
  North America
• Volume:
  25
• Issue:
  8
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha-512:4a1adab3176c83b96ac0ff7b9473b63dd38da59c7f9b203135c4886edd156bdc5807187fe8afd6599ddd0d5daefef5c744fcc45072b4f0e0510b2009436197d1
• Download URL:
  https://stacks.cdc.gov/view/cdc/80321/cdc_80321_DS1.pdf
• File Type:
  [PDF - 2.75 MB ]