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Body mass index in relation to extracellular vesicle linked microRNAs in human follicular fluid
  • Published Date:
    May 27 2019
  • Source:
    Fertil Steril. 112(2):387-396.e3
  • Language:
    English


Public Access Version Available on: August 01, 2020 information icon
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Details:
  • Pubmed ID:
    31146888
  • Pubmed Central ID:
    PMC6663626
  • Description:
    Objective:

    To study whether increased body mass index is associated with altered expression of extracellular vesicle microRNAs (EV-linked miRNAs) in human follicular fluid.

    Design:

    A cross-sectional study

    Setting:

    Tertiary, university-affiliated center

    Patients:

    One hundred and thirty-three women undergoing in vitro fertilization (IVF) were recruited between January 2014 and August 2016.

    Interventions:

    None

    Main Outcome Measures:

    EV-linked miRNAs were isolated from follicular fluid and their expression profiles were measured using the TaqMan Open Array® Human miRNA panel. EV-linked miRNAs were globally normalized and inverse-normal transformed. Associations between BMI and EV-linked miRNA outcomes were analyzed using multi-variate linear regression and principal component analysis.

    Result(s):

    Eighteen EV-linked miRNAs were associated with an increase in BMI after adjusting for age, ethnicity, smoking status and batch effects. Hsa-miR-328 remained significant after false-discovery rate adjustments. Principal component analyses identified the first principal component to account for 40% of the variation in our EV-linked miRNA dataset and adjusted linear regression found the first principal component was significantly associated with BMI after multiple testing adjustments. Using Kyoto Encyclopedia of Genes and Genomes enrichment analyses, we predicted gene targets of EV-linked miRNA in silico and identified PI3K-Akt signaling, ECM-receptor interaction, focal adhesion, FoxO signaling, and oocyte meiosis pathways.

    Conclusions:

    These results show that a one-unit increase in BMI is associated with altered follicular fluid expression of EV-linked miRNAs that may influence follicular and oocyte developmental pathways. Our findings provide potential insight into a mechanistic explanation for the reduced fertility rates associated with increased BMI.

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