In vitro human skin permeation of benzene in gasoline: Effects of concentration, multiple dosing and skin preparation

Details

• Alternative Title:
  J Expo Sci Environ Epidemiol
• Personal Author:
  Frasch, H. Frederick ; Barbero, Ana M.
• Description:
  In vitro human skin benzene permeation was measured from gasoline formulations with benzene concentrations ranging from 0.8 to 10 vol% and from neat benzene. Steady-state fluxes (J|), permeability coefficients (k|) and lag times (t|) were calculated from infinite dose exposures. Permeation of benzene from small gasoline doses administered over a two-day period was also studied. The thermodynamic activity of benzene in gasoline at 30 °C was determined and the solution is near-ideal over the range from 0.8 to 100 vol%. J| through human epidermal membranes were linear (R|=0.92) with concentration over the range from 0.8 to 10 vol%. J| (μg/cm|/h) from gasoline (0.8 vol% benzene=6.99 mg/ml) through epidermis and full-thickness skin were 9.37±1.41 and 1.82±0.44, respectively. Neat benzene J| was 566±138. Less than 0.25% of the total applied benzene mass from finite doses (10 μl/cm|) of gasoline was detected in receptor cells, and a small reduction of barrier function was observed from six total doses administered over 2 days. Application of these results to dermal exposure assessment examples demonstrates a range of systemic benzene uptakes that can be expected from occupational and consumer dermal exposures to gasoline, depending on the type and extent of exposure.
• Subjects:
  Adult Analysis Of Variance Benzene Chromatography, Gas Environmental Exposure Female Gasoline Humans In Vitro Techniques Mammaplasty Permeability Skin Skin Absorption Tissue Banks West Virginia Young Adult

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• Keywords:
  Activity Coefficie Dermal Risk Assessment Finite Dose Fractional Absorption Infinite Dose Lag Time
• Source:
  J Expo Sci Environ Epidemiol. 28(2):193-201
• Pubmed ID:
  28792002
• Pubmed Central ID:
  PMC6531855
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHSUnited States/
• Volume:
  28
• Issue:
  2
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha-512:28292cc871c456f64b1dad8f50de6ee2eb4d6b425ce43d1422408638779160d3845ad5d38c700983292c903d37fa092a7220f0e0e8afeaf68fc24de930ebd965
• Download URL:
  https://stacks.cdc.gov/view/cdc/79010/cdc_79010_DS1.pdf
• File Type:
  [PDF - 817.95 KB ]