Multicenter Study of Cronobacter sakazakii Infections in Humans, Europe, 2017

Details

• Alternative Title:
  Emerg Infect Dis
• Personal Author:
  Lepuschitz, Sarah ; Ruppitsch, Werner ; Pekard-Amenitsch, Shiva ; Forsythe, Stephen J. ; Cormican, Martin ; Mach, Robert L. ; Piérard, Denis ; Allerberger, Franz
• Corporate Authors:
  EUCRONI Study Group
• Description:
  Cronobacter sakazakii has been documented as a cause of life-threating infections, predominantly in neonates. We conducted a multicenter study to assess the occurrence of C. sakazakii across Europe and the extent of clonality for outbreak detection. National coordinators representing 24 countries in Europe were requested to submit all human C. sakazakii isolates collected during 2017 to a study center in Austria. Testing at the center included species identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, subtyping by whole-genome sequencing (WGS), and determination of antimicrobial resistance. Eleven countries sent 77 isolates, including 36 isolates from 2017 and 41 historical isolates. Fifty-nine isolates were confirmed as C. sakazakii by WGS, highlighting the challenge of correctly identifying Cronobacter spp. WGS-based typing revealed high strain diversity, indicating absence of multinational outbreaks in 2017, but identified 4 previously unpublished historical outbreaks. WGS is the recommended method for accurate identification, typing, and detection of this pathogen.
• Subjects:
  Anti-Bacterial Agents Cronobacter Sakazakii Drug Resistance, Bacterial Enterobacteriaceae Infections/Epidemiology Enterobacteriaceae Infections/History Enterobacteriaceae Infections/Microbiology Genome, Bacterial Genomics History, 21St Century Humans Infant, Newborn Male Microbial Sensitivity Tests Molecular Typing Multilocus Sequence Typing Phylogeny Public Health Surveillance Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Whole Genome Sequencing

  More +
• Source:
  Emerg Infect Dis. 25(3):515-522
• Pubmed ID:
  30789137
• Pubmed Central ID:
  PMC6390735
• Document Type:
  Journal Article
• Genre:
  Historical Article
• Place as Subject:
  Europe
• Volume:
  25
• Issue:
  3
• Collection(s):
  Emerging Infectious Diseases
• Main Document Checksum:
  urn:sha256:c8e87620c2aed2057ce765fb39389c9a44f26e2b8edb04a1b1d035e460ab3a97
• Download URL:
  https://stacks.cdc.gov/view/cdc/76668/cdc_76668_DS1.pdf
• File Type:
  [PDF - 1.67 MB ]