Genomic Characterization of β-Glucuronidase–Positive Escherichia coli O157:H7 Producing Stx2a

Ogura, Yoshitoshi; Seto, Kazuko; Morimoto, Yo; Nakamura, Keiji; Sato, Mitsuhiko P.; Gotoh, Yasuhiro; Itoh, Takehiko; Toyoda, Atsushi; Ohnishi, Makoto; Hayashi, Tetsuya

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Genomic Characterization of β-Glucuronidase–Positive Escherichia coli O157:H7 Producing Stx2a

Supporting Files Public Domain

December 2018
By Ogura, Yoshitoshi ; Seto, Kazuko ; Morimoto, Yo ; ...

File Language:

English

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Alternative Title:

Emerg Infect Dis
Personal Author:

Ogura, Yoshitoshi ; Seto, Kazuko ; Morimoto, Yo ; Nakamura, Keiji ; Sato, Mitsuhiko P. ; Gotoh, Yasuhiro ; Itoh, Takehiko ; Toyoda, Atsushi ; Ohnishi, Makoto ; Hayashi, Tetsuya
Description:

Among Shiga toxin (Stx)-producing Escherichia coli (STEC) O157:H7 strains, those producing Stx2a cause more severe diseases. Atypical STEC O157:H7 strains showing a β-glucuronidase-positive phenotype (GP STEC O157:H7) have rarely been isolated from humans, mostly from persons with asymptomatic or mild infections; Stx2a-producing strains have not been reported. We isolated, from a patient with bloody diarrhea, a GP STEC O157:H7 strain (PV15-279) that produces Stx2a in addition to Stx1a and Stx2c. Genomic comparison with other STEC O157 strains revealed that PV15-279 recently emerged from the stx1a/stx2c-positive GP STEC O157:H7 clone circulating in Japan. Major virulence genes are shared between typical (β-glucuronidase-negative) and GP STEC O157:H7 strains, and the Stx2-producing ability of PV15-279 is comparable to that of typical STEC O157:H7 strains; therefore, PV15-279 presents a virulence potential similar to that of typical STEC O157:H7. This study reveals the importance of GP O157:H7 as a source of highly pathogenic STEC clones.
Subjects:

Bacteria Enteric Infections Escherichia Coli Genome Genomic Characterization Of β-Glucuronidase–Positive Escherichia Coli O157:H7 Producing Stx2a GP STEC O157:H7 Research Shiga Toxin STEC Stx2a β-glucuronidase
Source:

Emerg Infect Dis. 24(12):2219-2227
Pubmed ID:

30457544
Pubmed Central ID:

PMC6256406
Document Type:

Journal Article
Volume:

24
Issue:

12
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:7e1b09c7bb9015ea061bbd83834fd22656cd23060202f7226eb7a9fac90e78cf
Download URL:

https://stacks.cdc.gov/view/cdc/61346/cdc_61346_DS1.pdf
File Type:

[PDF - 2.97 MB ]

File Language:

English

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