Details:

Alternative Title:
J Anal Toxicol
Personal Author:
Isenberg, Samantha L. ; Carter, Melissa D. ; Miller, Michael A. ; Noras, Aleksandra I. ; Mojica, Mike A. ; ... More +
Isenberg, Samantha L. ; Carter, Melissa D. ; Miller, Michael A. ; Noras, Aleksandra I. ; Mojica, Mike A. ; Carlson, Sean T. ; Bulathsinghala, Chinthaka P. ; Thomas, Jerry D. ; Johnson, Rudolph C. Less -
Description:
Ricin and abrin are toxic ribosome-inactivating proteins found in plants. Exposure to these toxins can be detected using the biomarkers ricinine and abrine, which are present in the same plant sources as the toxins. The concentration of the biomarkers in urine and blood will be dependent upon the purification of abrin or ricin, the route of exposure, and the length of time between exposure and sample collection. Here, we present the first diagnostic assay for the simultaneous quantification of both ricinine and abrine in blood matrices. Furthermore, this is the first-ever method for the detection of abrine in blood products. Samples were processed by isotope-dilution, solid-phase extraction, protein precipitation and quantification by HPLC-MS-MS. This analytical method detects abrine from 5.00 to 500 ng/mL and ricinine from 0.300 to 300 ng/mL with coefficients of determination of 0.996 ± 0.003 and 0.998 ± 0.002 (n = 22), respectively. Quality control material accuracy was determined to have <10% relative error, and precision was within 19% relative standard deviation. The assay's time-to-first result is three hours including sample preparation. Furthermore, the method was applied for the quantification of ricinine in the blood of a patient who had intentionally ingested castor beans to demonstrate the test was fit-for-purpose. This assay was designed to support the diagnosis of ricin and abrin exposures in public health investigations.
Subjects:
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Abrin Abrine Alkaloids Article Biomarkers Calibration Forensic Toxicology Humans Indole Alkaloids Isotope Dilution Limit Of Detection Poisoning Pyridones Quantitative HPLC-MS/MS Reproducibility Of Results Ricin Ricinine Specimen Handling
Source:
J Anal Toxicol. 42(9):630-636
Pubmed ID:
29931062
Pubmed Central ID:
PMC6214751
Document Type:
Journal Article
Funding:
CC999999/Intramural CDC HHS/United States
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:38b263496fdeab8a3e0c5bb37919b8c4c1e9acaf729ee9b9b8a331becfa08700
Download URL:
https://stacks.cdc.gov/view/cdc/60320/cdc_60320_DS1.pdf
File Type:

Supporting Files

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