From Culturomics to Clinical Microbiology and Forward

Dubourg, Grégory; Baron, Sophie; Cadoret, Frédéric; Couderc, Carine; Fournier, Pierre-Edouard; Lagier, Jean-Christophe; Raoult, Didier

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From Culturomics to Clinical Microbiology and Forward

Supporting Files Public Domain

Sep 2018
By Dubourg, Grégory ; Baron, Sophie ; Cadoret, Frédéric ; ...

File Language:

English

Details

Alternative Title:

Emerg Infect Dis
Personal Author:

Dubourg, Grégory ; Baron, Sophie ; Cadoret, Frédéric ; Couderc, Carine ; Fournier, Pierre-Edouard ; Lagier, Jean-Christophe ; Raoult, Didier
Description:

Culturomics has permitted discovery of hundreds of new bacterial species isolated from the human microbiome. Profiles generated by using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry have been added to the mass spectrometer database used in clinical microbiology laboratories. We retrospectively collected raw data from MALDI-TOF mass spectrometry used routinely in our laboratory in Marseille, France, during January 2012-March 2018 and analyzed 16S rDNA sequencing results from misidentified strains. During the study period, 744 species were identified from clinical specimens, of which 21 were species first isolated from culturomics. This collection involved 105 clinical specimens, accounting for 98 patients. In 64 cases, isolation of the bacteria was considered clinically relevant. MALDI-TOF mass spectrometry was able to identify the species in 95.2% of the 105 specimens. While contributing to the extension of the bacterial repertoire associated with humans, culturomics studies also enlarge the spectrum of prokaryotes involved in infectious diseases.
Subjects:

Bacteria Clinical Microbiology Culture Culturomics From Culturomics To Clinical Microbiology And Forward MALDI-TOF MS Matrix-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry Microbiology New Species Research
Source:

Emerg Infect Dis. 24(9):1683-1690.
Pubmed ID:

30124405
Pubmed Central ID:

PMC6106433
Document Type:

Journal Article
Volume:

24
Issue:

9
Collection(s):

Emerging Infectious Diseases
Main Document Checksum:

urn:sha256:31469ee955716df4dc65184c28c8eb915d14eb92619c7bb6ee35c74c0286a91c
Download URL:

https://stacks.cdc.gov/view/cdc/58617/cdc_58617_DS1.pdf
File Type:

[PDF - 669.77 KB ]

File Language:

English

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