Direct Detection of Carbapenem-Resistant Organisms from Environmental Samples Using the GeneXpert Molecular Diagnostic System

Perry, K. A.; Daniels, J. B.; Reddy, S. C.; Kallen, A. J.; Halpin, A. L.; Rasheed, J. K.; Noble-Wang, J. A.

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Direct Detection of Carbapenem-Resistant Organisms from Environmental Samples Using the GeneXpert Molecular Diagnostic System

Published Date:

Aug 01 2018

Source:

mSphere. 3(4).

Language:

English

[PDF-171.28 KB]

Details:

Personal Authors:

Perry, K. A. ; Daniels, J. B. ; Reddy, S. C. ; Kallen, A. J. ; Halpin, A. L. ; ... More ▼

Keywords:

[+]

Pubmed ID:

30068556

Pubmed Central ID:

PMC6070738

Description:

In this pilot study, traditional culture and PCR methods were compared to the Cepheid GeneXpert IV molecular diagnostic system with the Xpert Carba-R assay (Carba-R assay) for detection of carbapenem resistance genes in primary environmental samples collected during a health care-related outbreak. Overall, traditional culture-dependent PCR and the Carba-R assay demonstrated 75% agreement. The Carba-R assay detected carbapenemase genes in five additional samples and in two samples that had additional genes when compared to culture-dependent PCR. The Carba-R assay could be useful for prioritizing further testing of environmental samples during health care-related outbreaks.| Use of the Carba-R assay for detection of carbapenem-resistant Gram-negative organisms (CROs) can provide data for implementation of a rapid infection control response to minimize the spread of CROs in the health care setting.

Document Type:

Journal Article

Collection(s):

CDC Public Access

Main Document Checksum:

[+]

Supporting Files:

mSphere.00113-18.nxml

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