Direct Detection of Carbapenem-Resistant Organisms from Environmental Samples Using the GeneXpert Molecular Diagnostic System

Details

• Alternative Title:
  mSphere
• Personal Author:
  Perry, K. A. ; Daniels, J. B. ; Reddy, S. C. ; Kallen, A. J. ; Halpin, A. L. ; Rasheed, J. K. ; Noble-Wang, J. A.
• Description:
  In this pilot study, traditional culture and PCR methods were compared to the Cepheid GeneXpert IV molecular diagnostic system with the Xpert Carba-R assay (Carba-R assay) for detection of carbapenem resistance genes in primary environmental samples collected during a health care-related outbreak. Overall, traditional culture-dependent PCR and the Carba-R assay demonstrated 75% agreement. The Carba-R assay detected carbapenemase genes in five additional samples and in two samples that had additional genes when compared to culture-dependent PCR. The Carba-R assay could be useful for prioritizing further testing of environmental samples during health care-related outbreaks.| Use of the Carba-R assay for detection of carbapenem-resistant Gram-negative organisms (CROs) can provide data for implementation of a rapid infection control response to minimize the spread of CROs in the health care setting.
• Subjects:
  Carbapenemase Genes Carbapenem Resistant Cepheid GeneXpert Observation PCR
• Source:
  mSphere. 3(4).
• Pubmed ID:
  30068556
• Pubmed Central ID:
  PMC6070738
• Document Type:
  Journal Article
• Volume:
  3
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:fb5d3e15986d8ab1cbc2cff1a51f9188b7b6cc472d714bfa3995b22fc84b7b33
• Download URL:
  https://stacks.cdc.gov/view/cdc/58234/cdc_58234_DS1.pdf
• File Type:
  [PDF - 171.28 KB ]