Details:

Alternative Title:
mSphere
Personal Author:
Perry, K. A. ; Daniels, J. B. ; Reddy, S. C. ; Kallen, A. J. ; Halpin, A. L. ; ... More +
Perry, K. A. ; Daniels, J. B. ; Reddy, S. C. ; Kallen, A. J. ; Halpin, A. L. ; Rasheed, J. K. ; Noble-Wang, J. A. ; Less -
Description:
In this pilot study, traditional culture and PCR methods were compared to the Cepheid GeneXpert IV molecular diagnostic system with the Xpert Carba-R assay (Carba-R assay) for detection of carbapenem resistance genes in primary environmental samples collected during a health care-related outbreak. Overall, traditional culture-dependent PCR and the Carba-R assay demonstrated 75% agreement. The Carba-R assay detected carbapenemase genes in five additional samples and in two samples that had additional genes when compared to culture-dependent PCR. The Carba-R assay could be useful for prioritizing further testing of environmental samples during health care-related outbreaks.| Use of the Carba-R assay for detection of carbapenem-resistant Gram-negative organisms (CROs) can provide data for implementation of a rapid infection control response to minimize the spread of CROs in the health care setting.
Subject:
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Carbapenemase Genes
Carbapenem Resistant
Cepheid GeneXpert
Observation
PCR

Source:
mSphere. 3(4). ;
Pubmed ID:
30068556
Pubmed Central ID:
PMC6070738
Document Type:
Journal Article ;
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:fb5d3e15986d8ab1cbc2cff1a51f9188b7b6cc472d714bfa3995b22fc84b7b33
File Type:

Supporting Files

• mSphere.00113-18.nxml

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