Details:

Alternative Title:
Nat Methods
Personal Author:
Gut, Gabriele ; Tadmor, Michelle D ; Pe’er, Dana ; Pelkmans, Lucas ; Liberali, Prisca
Gut, Gabriele ; Tadmor, Michelle D ; Pe’er, Dana ; Pelkmans, Lucas ; Liberali, Prisca Less -
Description:
An accurate dissection of sources of cell-to-cell variability is crucial for quantitative biology at the single-cell level but has been challenging for the cell cycle. We present Cycler, a robust method that constructs a continuous trajectory of cell-cycle progression from images of fixed cells. Cycler handles heterogeneous microenvironments and does not require perturbations or genetic markers, making it generally applicable to quantifying multiple sources of cell-to-cell variability in mammalian cells.
Subject:
[+]
Article Cell Cycle Cell Proliferation Cyclin A DNA Replication Glycogen Synthase Kinase 3 HeLa Cells Humans Image Processing, Computer-Assisted Proliferating Cell Nuclear Antigen Reproducibility Of Results Single-Cell Analysis Support Vector Machine Tubulin
Source:
Nat Methods. 12(10):951-954.
Pubmed ID:
26301842
Pubmed Central ID:
PMC6004611
Document Type:
Journal Article
Funding:
DP1 HD084071/HD/NICHD NIH HHS/United States ; DP1-HD084071/DP/NCCDPHP CDC HHS/United States
DP1 HD084071/HD/NICHD NIH HHS/United States ; DP1-HD084071/DP/NCCDPHP CDC HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:469dca1e817dd2db0228f14a4f2d582b6cfcf92c2d93dc6ae1ca2ae934b5b21d
Download URL:
https://stacks.cdc.gov/view/cdc/56157/cdc_56157_DS1.pdf
File Type:

Supporting Files

• 	NIHMS972075-supplement-Supplemental.pdf	pdf
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