Trajectories of cell-cycle progression from fixed cell populations

Details

• Alternative Title:
  Nat Methods
• Personal Author:
  Gut, Gabriele ; Tadmor, Michelle D ; Pe’er, Dana ; Pelkmans, Lucas ; Liberali, Prisca
• Description:
  An accurate dissection of sources of cell-to-cell variability is crucial for quantitative biology at the single-cell level but has been challenging for the cell cycle. We present Cycler, a robust method that constructs a continuous trajectory of cell-cycle progression from images of fixed cells. Cycler handles heterogeneous microenvironments and does not require perturbations or genetic markers, making it generally applicable to quantifying multiple sources of cell-to-cell variability in mammalian cells.
• Subjects:
  Article Cell Cycle Cell Proliferation Cyclin A DNA Replication Glycogen Synthase Kinase 3 HeLa Cells Humans Image Processing, Computer-Assisted Proliferating Cell Nuclear Antigen Reproducibility Of Results Single-Cell Analysis Support Vector Machine Tubulin

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• Source:
  Nat Methods. 12(10):951-954.
• Pubmed ID:
  26301842
• Pubmed Central ID:
  PMC6004611
• Document Type:
  Journal Article
• Funding:
  DP1 HD084071/HD/NICHD NIH HHS/United States ; DP1-HD084071/DP/NCCDPHP CDC HHS/United States
• Volume:
  12
• Issue:
  10
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:469dca1e817dd2db0228f14a4f2d582b6cfcf92c2d93dc6ae1ca2ae934b5b21d
• Download URL:
  https://stacks.cdc.gov/view/cdc/56157/cdc_56157_DS1.pdf
• File Type:
  [PDF - 731.54 KB ]