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Trajectories of cell-cycle progression from fixed cell populations
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Aug 24 2015
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Source: Nat Methods. 12(10):951-954.
[PDF-731.54 KB]
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Alternative Title:Nat Methods
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Description:An accurate dissection of sources of cell-to-cell variability is crucial for quantitative biology at the single-cell level but has been challenging for the cell cycle. We present Cycler, a robust method that constructs a continuous trajectory of cell-cycle progression from images of fixed cells. Cycler handles heterogeneous microenvironments and does not require perturbations or genetic markers, making it generally applicable to quantifying multiple sources of cell-to-cell variability in mammalian cells.
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Pubmed ID:26301842
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Pubmed Central ID:PMC6004611
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Volume:12
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Issue:10
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