Details:

Alternative Title:
Extremophiles
Personal Author:
Pan, Miao ; Santangelo, Thomas J. ; Čuboňová, Ľbomíra ; Li, Zhuo ; Metangmo, Harlette ; ... More +
Pan, Miao ; Santangelo, Thomas J. ; Čuboňová, Ľbomíra ; Li, Zhuo ; Metangmo, Harlette ; Ladner, Jane ; Hurwitz, Jerard ; Reeve, John N. ; Kelman, Zvi Less -
Description:
Proliferating cell nuclear antigen (PCNA) monomers assemble to form a ring-shaped clamp complex that encircles duplex DNA. PCNA binding to other proteins tethers them to the DNA providing contacts and interactions for many other enzymes essential for DNA metabolic processes. Most eukarya and euryarchaea have only one PCNA homolog but Thermococcus kodakarensis uniquely has two, designated PCNA1 and PCNA2, encoded by TK0535 and TK0582, respectively. Here, we establish that both PCNA1 and PCNA2 form homotrimers that stimulate DNA synthesis by archaeal DNA polymerases B and D and ATP hydrolysis by the replication factor C complex. In exponentially growing cells, PCNA1 is abundant and present at an ~100-fold higher concentration than PCNA2 monomers. Deletion of TK0582 (PCNA2) had no detectable effects on viability or growth whereas repeated attempts to construct a T. kodakarensis strain with TK0535 (PCNA1) deleted were unsuccessful. The implications of these observations for PCNA1 function and the origin of the two PCNA-encoding genes in T. kodakarensis are discussed.
Subjects:
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Archaea Archaeal Proteins Article DNA Replication Gene Deletion Genetics Microbial Viability PCNA Proliferating Cell Nuclear Antigen Protein Multimerization Structure Thermococcus Thermococcus Kodakarensis
Source:
Extremophiles. 2013; 17(3):453-461.
Document Type:
Journal Article
Funding:
GH034559/GH/CGH CDC HHS/United States ; GM073336/GM/NIGMS NIH HHS/United States ; GM53185/GM/NIGMS NIH HHS/United States ; R01 GM053185/GM/NIGMS NIH HHS/United States
GH034559/GH/CGH CDC HHS/United States ; GM073336/GM/NIGMS NIH HHS/United States ; GM53185/GM/NIGMS NIH HHS/United States ; R01 GM053185/GM/NIGMS NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:56e2cdff8466ffb620724622fd8550300e460503492e336a84d2767ade47bef9
Download URL:
https://stacks.cdc.gov/view/cdc/29706/cdc_29706_DS1.pdf
File Type:

Supporting Files

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  	NIHMS507958-supplement-Supplementary_data_2.pdf	pdf
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