DNA microarray-based assessment of virulence potential of Shiga toxin gene-carrying Escherichia coli O104:H7 isolated from feedlot cattle feces

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• Alternative Title:
  PLoS One
• Personal Author:
  Shridhar, Pragathi B. ; Patel, Isha R. ; Gangiredla, Jayanthi ; Noll, Lance W. ; Shi, Xiaorong ; Bai, Jianfa ; Elkins, Christopher A. ; Strockbine, Nancy ; Nagaraja, T. G.
• Description:
  Escherichia coli O104:H4, a hybrid pathotype reported in a large 2011 foodborne outbreak in Germany, has not been detected in cattle feces. However, cattle harbor and shed in the feces other O104 serotypes, particularly O104:H7, which has been associated with sporadic cases of diarrhea in humans. The objective of our study was to assess the virulence potential of Shiga toxin-producing E. coli (STEC) O104:H7 isolated from feces of feedlot cattle using DNA microarray. Six strains of STEC O104:H7 isolated from cattle feces were analyzed using FDA-E. coli Identification (ECID) DNA microarray to determine their virulence profiles and compare them to the human strains (clinical) of O104:H7, STEC O104:H4 (German outbreak strain), and O104:H21 (milk-associated Montana outbreak strain). Scatter plots were generated from the array data to visualize the gene-level differences between bovine and human O104 strains, and Pearson correlation coefficients (r) were determined. Splits tree was generated to analyze relatedness between the strains. All O104:H7 strains, both bovine and human, similar to O104:H4 and O104:H21 outbreak strains were negative for intimin (eae). The bovine strains were positive for Shiga toxin 1 subtype c (stx1c), enterohemolysin (ehxA), tellurite resistance gene (terD), IrgA homolog protein (iha), type 1 fimbriae (fimH), and negative for genes that code for effector proteins of type III secretory system. The six cattle O104 strains were closely related (r = 0.86-0.98) to each other, except for a few differences in phage related and non-annotated genes. One of the human clinical O104:H7 strains (2011C-3665) was more closely related to the bovine O104:H7 strains (r = 0.81-0.85) than the other four human clinical O104:H7 strains (r = 0.75-0.79). Montana outbreak strain (O104:H21) was more closely related to four of the human clinical O104:H7 strains than the bovine O104:H7 strains. None of the bovine E. coli O104 strains carried genes characteristic of E. coli O104:H4 German outbreak strain and unlike other human strains were also negative for Shiga toxin 2. Because cattle E. coli O104:H7 strains possess stx1c and genes that code for enterohemolysin and a variety of adhesins, the serotype has the potential to be a diarrheagenic foodborne pathogen in humans.
• Subjects:
  Adhesins, Bacterial Animals Cattle Disease Outbreaks Escherichia Coli Infections Escherichia Coli O104 Escherichia Coli Proteins Feces Genotype Hemolysin Proteins Humans Models, Statistical Oligonucleotide Array Sequence Analysis Phenotype Phylogeny Serotyping Shiga-Toxigenic Escherichia Coli Shiga Toxin Virulence

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• Source:
  PLoS One. 13(4).
• Pubmed ID:
  29708991
• Pubmed Central ID:
  PMC5927410
• Document Type:
  Journal Article
• Volume:
  13
• Issue:
  4
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:416fcf58dd18d68c43588fcda59e9fd2ac3d7e0e34375cf8d07b54c091fc7052
• Download URL:
  https://stacks.cdc.gov/view/cdc/55643/cdc_55643_DS1.pdf
• File Type:
  [PDF - 1.06 MB ]