Parallel evolution of vgsc mutations at domains IS6, IIS6 and IIIS6 in pyrethroid resistant Aedes aegypti from Mexico

Details

• Alternative Title:
  Sci Rep
• Personal Author:
  Saavedra-Rodriguez, Karla ; Maloof, Farah Vera ; Campbell, Corey L. ; Garcia-Rejon, Julian ; Lenhart, Audrey ; Penilla, Patricia ; Rodriguez, Americo ; Sandoval, Arturo Acero ; Flores, Adriana E. ; Ponce, Gustavo ; Lozano, Saul ; Black, William C.
• Description:
  Aedes aegypti is the primary urban mosquito vector of viruses causing dengue, Zika and chikungunya fevers -for which vaccines and effective pharmaceuticals are still lacking. Current strategies to suppress arbovirus outbreaks include removal of larval-breeding sites and insecticide treatment of larval and adult populations. Insecticidal control of Ae. aegypti is challenging, due to a recent rapid global increase in knockdown-resistance (kdr) to pyrethroid insecticides. Widespread, heavy use of pyrethroid space-sprays has created an immense selection pressure for kdr, which is primarily under the control of the voltage-gated sodium channel gene (vgsc). To date, eleven replacements in vgsc have been discovered, published and shown to be associated with pyrethroid resistance to varying degrees. In Mexico, F1,534C and V1,016I have co-evolved in the last 16 years across Ae. aegypti populations. Recently, a novel replacement V410L was identified in Brazil and its effect on vgsc was confirmed by electrophysiology. Herein, we screened V410L in 25 Ae. aegypti historical collections from Mexico, the first heterozygote appeared in 2002 and frequencies have increased in the last 16 years alongside V1,016I and F1,534C. Knowledge of the specific vgsc replacements and their interaction to confer resistance is essential to predict and to develop strategies for resistance management.
• Subjects:
  Aedes Animals Chikungunya Fever Dengue Insecticide Resistance Insecticides Insect Proteins Mutation Protein Domains Pyrethrins Zika Virus

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• Source:
  Sci Rep. 8.
• Pubmed ID:
  29712956
• Pubmed Central ID:
  PMC5928250
• Document Type:
  Journal Article
• Funding:
  D43 TW001130/TW/FIC NIH HHS/United States ; R01 AI121211/AI/NIAID NIH HHS/United States ; U01 AI088647/AI/NIAID NIH HHS/United States
• Place as Subject:
  Brazil ; Mexico
• Volume:
  8
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:dab229e44105fcee7dde8e5b40daf1279fea4558ed3c91f0858310ef0116f7d7
• Download URL:
  https://stacks.cdc.gov/view/cdc/55265/cdc_55265_DS1.pdf
• File Type:
  [PDF - 1.45 MB ]