Inducing Stem Cell Myogenesis Using NanoScript

Details

• Alternative Title:
  ACS Nano
• Personal Author:
  Patel, Sahishnu ; Yin, Perry T. ; Sugiyama, Hiroshi ; Lee, Ki-Bum
• Description:
  Transcription factors (TFs) are multidomain proteins that play a critical role in orchestrating stem cell differentiation, but several limitations hinder the full potential of TF-based gene regulation. Here we report a unique strategy to emulate TFs and differentiate stem cells in a nonviral approach using an artificial, nanoparticle-based transcription factor called NanoScript. The NanoScript platform consists of a gold nanoparticle functionalized with small molecules that mimic the various domains of TFs. As a result, NanoScript mimics the function and structure of TF proteins. Specifically, NanoScript was designed to regulate muscle cell differentiation by targeting myogenic regulatory factors (MRFs), which play an important role in inducing myogenesis. This NanoScript-MRF is stable in physiological environments, localizes within the nucleus, induces differentiation of adipose-derived mesenchymal stem cells into mature muscle cells in 7 days, and is naturally excreted from induced muscle cells. As such, NanoScript represents a safe and powerful tool for applications requiring gene manipulation.
• Subjects:
  Cell Differentiation Cell Line Gold Humans Mesenchymal Stem Cells Metal Nanoparticles Muscle Cells Muscle Development Myogenic Regulatory Factors
• Keywords:
  Myogenesis Nanoparticle-based Genetic Manipulation Nonviral Delivery Stem Cell Differentiation Transcription Factors
• Source:
  ACS Nano. 9(7):6909-6917
• Pubmed ID:
  26108385
• Pubmed Central ID:
  PMC5808887
• Document Type:
  Journal Article
• Funding:
  DP2 OD006462/OD/NIH HHSUnited States/ ; R21 NS085569/NS/NINDS NIH HHSUnited States/ ; 1DP20D006462-01/DP/NCCDPHP CDC HHSUnited States/ ; 1R21NS085569-01/NS/NINDS NIH HHSUnited States/
• Volume:
  9
• Issue:
  7
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:def8b084d59fae7940fad20f6d3a42188e410b1a7495388b1aaff8c3673312dc
• Download URL:
  https://stacks.cdc.gov/view/cdc/51681/cdc_51681_DS1.pdf
• File Type:
  [PDF - 1.19 MB ]