NanoScript: A Nanoparticle-Based Artificial Transcription Factor for Effective Gene Regulation

Details

• Alternative Title:
  ACS Nano
• Personal Author:
  Patel, Sahishnu ; Jung, Dongju ; Yin, Perry T. ; Carlton, Peter ; Yamamoto, Makoto ; Bando, Toshikazu ; Sugiyama, Hiroshi ; Lee, Ki-Bum
• Description:
  Transcription factor (TF) proteins are master regulators of transcriptional activity and gene expression. TF-based gene regulation is a promising approach for many biological applications; however, several limitations hinder the full potential of TFs. Herein, we developed an artificial, nanoparticle-based transcription factor, termed NanoScript, which is designed to mimic the structure and function of TFs. NanoScript was constructed by tethering functional peptides and small molecules called synthetic transcription factors, which mimic the individual TF domains, onto gold nanoparticles. We demonstrate that NanoScript localizes within the nucleus and initiates transcription of a reporter plasmid by over 15-fold. Moreover, NanoScript can effectively transcribe targeted genes on endogenous DNA in a nonviral manner. Because NanoScript is a functional replica of TF proteins and a tunable gene-regulating platform, it has great potential for various stem cell applications.
• Subjects:
  Article Gene Activation Nanoparticle-based Genetic Manipulation Nonviral Delivery Synthetic Transcription Factors Transcription Factor Proteins
• Source:
  ACS Nano. 2014; 8(9):8959-8967.
• Pubmed ID:
  25133310
• Pubmed Central ID:
  PMC4174092
• Document Type:
  Journal Article
• Funding:
  1DP20D006462-01/DP/NCCDPHP CDC HHS/United States ; 1R21NS085569-01/NS/NINDS NIH HHS/United States
• Volume:
  8
• Issue:
  9
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:ac0e32fae6fae3d5763f7900f58d617e18fdd08c602bc73ed2548747500086e7
• Download URL:
  https://stacks.cdc.gov/view/cdc/32987/cdc_32987_DS1.pdf
• File Type:
  [PDF - 6.11 MB ]