Details:

Alternative Title:
ACS Nano
Publisher's site:
http://dx.doi.org/10.1021/acsnano.5b00641
Personal Author:
Lai, Jinping ; Shah, Birju P. ; Zhang, Yixiao ; Yang, Letao ; Lee, Ki-Bum
Lai, Jinping ; Shah, Birju P. ; Zhang, Yixiao ; Yang, Letao ; Lee, Ki-Bum Less -
Description:
Stimuli-responsive drug delivery vehicles have garnered immense interest in recent years due to unparalleled progress made in material science and nanomedicine. However, the development of stimuli-responsive devices with integrated real-time monitoring capabilities is still in its nascent stage because of the limitations of imaging modalities. In this paper, we describe the development of a polypeptide-wrapped mesoporous-silica-coated multicolor upconversion nanoparticle (UCNP@MSN) as an adenosine triphosphate (ATP)-responsive drug delivery system (DDS) for long-term tracking and real-time monitoring of drug release. Our UCNP@MSN with multiple emission peaks in UV-NIR wavelength range was functionalized with zinc-dipicolylamine analogue (TDPA-Zn(2+)) on its exterior surface and loaded with small-molecule drugs like chemotherapeutics in interior mesopores. The drugs remained entrapped within the UCNP-MSNs when the nanoparticles were wrapped with a compact branched polypeptide, poly(Asp-Lys)-b-Asp, because of multivalent interactions between Asp moieties present in the polypeptide and the TDPA-Zn(2+) complex present on the surface of UCNP-MSNs. This led to luminescence resonance energy transfer (LRET) from the UCNPs to the entrapped drugs, which typically have absorption in UV-visible range, ultimately resulting in quenching of UCNP emission in UV-visible range while retaining their strong NIR emission. Addition of ATP led to a competitive displacement of the surface bound polypeptide by ATP due to its higher affinity to TDPA-Zn(2+), which led to the release of the entrapped drugs and subsequent elimination of LRET. Monitoring of such ATP-triggered ratiometric changes in LRET allowed us to monitor the release of the entrapped drugs in real-time. Given these results, we envision that our proposed UCNP@MSN-polypeptide hybrid nanoparticle has great potential for stimuli-responsive drug delivery as well as for monitoring biochemical changes taking place in live cancer and stem cells.
Subject:
[+]
Adenosine Triphosphate
Amines
Article
Color
Core–shell Nanoparticles
Doxorubicin
Drug Carriers
Drug Liberation
Energy Transfer
Humans
Luminescence Resonance Energy Transfer (LRET)
MCF-7 Cells
Nanomedicine
Nanoparticles
Oligopeptides
Picolinic Acids
Porosity
Real-time Monitoring
Silicon Dioxide
Stimuli-responsive Drug Delivery
Upconversion Nanoparticle
Zinc

Source:
ACS Nano. 9(5):5234-5245.
Pubmed ID:
25859611
Pubmed Central ID:
PMC5808884
Document Type:
Journal Article
Funding:
DP2 OD006462/OD/NIH HHS/United States ; R21 NS085569/NS/NINDS NIH HHS/United States ; 1DP20D00646201/DP/NCCDPHP CDC HHS/United States ; 1R21NS085569-01/NS/NINDS NIH HHS/United States
DP2 OD006462/OD/NIH HHS/United States ; R21 NS085569/NS/NINDS NIH HHS/United States ; 1DP20D00646201/DP/NCCDPHP CDC HHS/United States ; 1R21NS085569-01/NS/NINDS NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:983ef67e64510d556d94a4d2ade3bcea377a474aa57252c7b5e15c606c0081ac
File Type:

Supporting Files

• 	nihms935920f4.gif	gif
  	nihms935920f4.jpg	jpeg
  	nihms935920f5.gif	gif
  	nihms935920f5.jpg	jpeg
  	nihms935920f6.gif	gif
  	nihms935920f6.jpg	jpeg
  	nihms935920u1.jpg	jpeg
  	NIHMS935920-supplement-SI.pdf	pdf
  	nihms935920.nxml	xml
  	nihms935920f1.gif	gif
  	nihms935920f1.jpg	jpeg
  	nihms935920f2.gif	gif
  	nihms935920f2.jpg	jpeg
  	nihms935920f3.gif	gif
  	nihms935920f3.jpg	jpeg

More +