Details:

Alternative Title:
ACS Nano
Publisher's site:
http://dx.doi.org/10.1021/nn400199t
Personal Author:
Lai, Jinping ; Shah, Birju P. ; Garfunkel, Eric ; Lee, Ki-Bum
Lai, Jinping ; Shah, Birju P. ; Garfunkel, Eric ; Lee, Ki-Bum Less -
Description:
We describe the development of a versatile fluorescence resonance energy transfer (FRET)-based real-time monitoring system, consisting of (a) coumarin-labeled-cysteine tethered mesoporous silica nanoparticles (MSNs) as the drug carrier, (b) a fluorescein isothiocyanate-β-cyclodextrin (FITC-β-CD) as redox-responsive molecular valve blocking the pores, and (c) a FRET donor-acceptor pair of coumarin and FITC integrated within the pore-unlocking event, thereby allowing for monitoring the release of drugs from the pores in real-time. Under nonreducing conditions, when the disulfide bond is intact, the close proximity between coumarin and FITC on the surface of MSNs results in FRET from coumarin to FITC. However, in the presence of the redox stimuli like glutathione (GSH), the disulfide bond is cleaved which leads to the removal of molecular valve (FITC-β-CD), thus triggering drug release and eliminating FRET. By engineering such a FRET-active donor-acceptor structure within the redox-responsive molecular valve, we can monitor the release of the drugs entrapped within the pores of the MSN nanocarrier, following the change in the FRET signal. We have demonstrated that, any exogenous or endogenous change in the GSH concentration will result in a change in the extent of drug release as well as a concurrent change in the FRET signal, allowing us to extend the applications of our FRET-based MSNs for monitoring the release of any type of drug molecule in real-time.
Subject:
[+]
Antineoplastic Agents
Article
Beta-Cyclodextrins
Computer Systems
Coumarins
Doxorubicin
Drug Carriers
Drug Delivery
Drug Delivery Systems
Drug Monitoring
Fluorescein-5-isothiocyanate
Fluorescence Resonance Energy Transfer
Glutathione
HeLa Cells
Humans
Mesoporous Silica Nanoparticle (MSN)-based Drug Delivery
Nanoparticles
Nanotechnology
Oxidation-Reduction
Real-time Monitoring
Silicon Dioxide
Stimuli-responsive
Fluorescence Resonance Energy Transfer

Source:
ACS Nano. 7(3):2741-2750.
Document Type:
Journal Article
Funding:
1DP20D006462-01/DP/NCCDPHP CDC HHS/United States ; DP2 OD006462/OD/NIH HHS/United States
1DP20D006462-01/DP/NCCDPHP CDC HHS/United States ; DP2 OD006462/OD/NIH HHS/United States Less -
Collection(s):
CDC Public Access
Main Document Checksum:
[+]
urn:sha256:d0dc8d3d0258f2d8884295e1d07cba172b8b44fab0b1b566c9a2eae4910da2b2
File Type:

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