Post-injury and resolution response to repetitive inhalation exposure to agricultural organic dust in mice

Details

• Alternative Title:
  Safety (Basel)
• Personal Author:
  Warren, Kristi J. ; Wyatt, Todd A. ; Romberger, Debra J. ; Ailts, Isaak ; West, William W. ; Nelson, Amy J. ; Nordgren, Tara M. ; Staab, Elizabeth ; Heires, Art J. ; Poole, Jill A.
• Description:
  Inhalation of organic dusts in agricultural environments causes airway inflammatory diseases. Despite advances in understanding the airway response to dust-induced inflammation, less is known about the transition from lung injury to repair and recovery. The objective of this study was to define the post-inflammation homeostasis events following organic dust-induced lung injury. Using an established protocol, mice were intranasally treated with swine confinement facility organic dust extract (ODE) daily for 3 weeks (repetitive exposure) or treated daily with ODE for 3 weeks followed by no treatment for 1-4 weeks (recovery period) whereupon lavage fluid, lung tissue, and sera were processed. During recovery period, a significant decrease was observed in ODE-induced neutrophil levels after 1 week, lymphocytes at 2 weeks, and macrophages at 4 weeks in the lavage fluid. ODE-induced lung cellular aggregates and bronchiolar compartment inflammation were diminished, but persisted for 4 weeks post-injury. Alveolar inflammation resolved at 3 weeks. ODE-induced lung neutrophils were cleared by 3 weeks, B-cells by 2 weeks, and CD3|CD4| and CD3|CD8| T cells by 4 week recovery period. Collectively, these results identify important processes during recovery period following agricultural dust-induced inflammation, and present possible strategies for improving lung repair and resolution.
• Subjects:
  Agriculture Article Farm Immunity Inflammation Lung Lymphocyte Neutrophil Repair Resolution Swine Barn

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• Source:
  Safety (Basel). 3(1)
• Pubmed ID:
  29387711
• Pubmed Central ID:
  PMC5788309
• Document Type:
  Journal Article
• Funding:
  IK6 BX003781/BX/BLRD VAUnited States/ ; U54OH010162/ACL/ACL HHSUnited States/ ; R01 OH008539/OH/NIOSH CDC HHSUnited States/ ; R01 ES019325/ES/NIEHS NIH HHSUnited States/ ; U54 OH010162/OH/NIOSH CDC HHSUnited States/ ; R00 ES025819/ES/NIEHS NIH HHSUnited States/ ; F32 ES022913/ES/NIEHS NIH HHSUnited States/
• Volume:
  3
• Issue:
  1
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:d61d91a2798e6c12c65e1ca6ced0d72f53db876c92f68a5f2ff038f552f2da3a
• Download URL:
  https://stacks.cdc.gov/view/cdc/51506/cdc_51506_DS1.pdf
• File Type:
  [PDF - 791.51 KB ]