Standard operating procedure for PulseNet PFGE of Vibrio cholera and Vibrio parahaemolyticus

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Standard operating procedure for PulseNet PFGE of Vibrio cholera and Vibrio parahaemolyticus

April 2013
By National Center for Emerging and Zoonotic Infectious Diseases (U.S.)

[PDF-733.79 KB]

English

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Alternative Title:

Pulsed-field Gel Electrophoresis
Corporate Authors:

National Center for Emerging and Zoonotic Infectious Diseases (U.S.)
Description:

Purpose: To describe the One-Day (24-26 hour) Standardized Laboratory Protocol for Molecular Subtyping of Vibrio cholera and Vibrio parahaemolyticus by Pulsed-field Gel Electrophoresis (PFGE).

Scope: To provide the PulseNet participants with the same procedure for performing PFGE of Vibrio cholerae and Vibrio parahaemolyticus, thus ensuring inter-laboratory comparability of the generated results.

PNL06 Last Updated April 2013

vibrio_pfge_protocol-508c.pdf
Subjects:

[+]

Bacterial Typing Techniques DNA, Bacterial Electrophoresis, Gel, Pulsed-Field Food Microbiology Vibrio Cholerae Vibrio Parahaemolyticus
Document Type:

Pamphlet (or booklet)
Collection(s):

Stephen B. Thacker CDC Library collection
Main Document Checksum:

[+]

urn:sha256:e2f4f6ac9cccc916f75cd7f79681de55efb19e5d4e048c81c32e716248821cc6
Download URL:

https://stacks.cdc.gov/view/cdc/51209/cdc_51209_DS1.pdf
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Standard operating procedure for PulseNet PFGE of Vibrio cholera and Vibrio parahaemolyticus

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