Ebola virus disease diagnostics, Sierra Leone: analysis of real-time RT-PCR values in clinical blood and oral swab specimens

Details

• Alternative Title:
  J Infect Dis
• Personal Author:
  Erickson, Bobbie R. ; Sealy, Tara K. ; Flietstra, Tim ; Morgan, Laura ; Kargbo, Brima ; Matt-Lebby, Victor E. ; Gibbons, Aridth ; Chakrabarti, Ayan K. ; Graziano, James ; Presser, Lance ; Flint, Mike ; Bird, Brian H. ; Brown, Shelley ; Klena, John D. ; Blau, Dianna M. ; Brault, Aaron C. ; Belser, Jessica A. ; Salzer, Johanna S. ; Schuh, Amy J. ; Lo, Michael ; Zivcec, Marko ; Priestley, Rachael A. ; Pyle, Meredith ; Goodman, Christin ; Bearden, Scott ; Amman, Brian R. ; Basile, Alison ; Bergeron, Éric ; Bowen, Michael D. ; Dodd, Kimberly A. ; Freeman, Molly M. ; McMullan, Laura K. ; Paddock, Christopher D. ; Russell, Brandy J. ; Sanchez, Angela J. ; Towner, Jonathan S. ; Wang, David ; Zemtsova, Galina E. ; Stoddard, Robyn A. ; Turnsek, Maryann ; Guerrero, Lisa Wiggleton ; Emery, Shannon L. ; Stovall, Janae ; Kainulainen, Markus H. ; Perniciaro, Jamie L. ; Mijatovic-Rustempasic, Slavica ; Shakirova, Gulchekhra ; Winter, Jörn ; Sexton, Christopher ; Liu, Feng ; Slater, Kimetha ; Anderson, Raydel ; Andersen, Lauren ; Chiang, Cheng-Feng ; Tzeng, Wen-Pin ; Crowe, Samuel J. ; Maenner, Matthew J. ; Spiropoulou, Christina F. ; Nichol, Stuart T. ; Ströher, Ute
• Description:
  During the Ebola virus outbreak of 2013-2016, the Viral Special Pathogens Branch field laboratory in Sierra Leone tested approximately 26 000 specimens between August 2014 and October 2015. Analysis of the B2M endogenous control Ct values showed its utility in monitoring specimen quality, comparing results with different specimen types, and interpretation of results. For live patients, blood is the most sensitive specimen type and oral swabs have little diagnostic utility. However, swabs are highly sensitive for diagnostic testing of corpses.
• Subjects:
  Clinical Laboratory Services Disease Outbreaks Ebolavirus Hemorrhagic Fever, Ebola Humans Laboratories Real-Time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction RNA, Viral Sensitivity And Specificity
• Source:
  J Infect Dis. 214(3):S258-S262.
• Pubmed ID:
  27587631
• Pubmed Central ID:
  PMC5769963
• Document Type:
  Journal Article
• Funding:
  CC999999/Intramural CDC HHS/United States
• Place as Subject:
  Sierra Leone
• Volume:
  214
• Issue:
  3
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:c4fb0a3268521c185fd73e342124c1816a96960ac898b3f91dd6ef7e492562e2
• Download URL:
  https://stacks.cdc.gov/view/cdc/51022/cdc_51022_DS1.pdf
• File Type:
  [PDF - 400.26 KB ]