Genomic characterization of Zika virus isolated from Indonesia

Details

• Alternative Title:
  Virology
• Personal Author:
  Yudhaputri, Frilasita A. ; Trimarsanto, Hidayat ; Perkasa, Aditya ; Yohan, Benediktus ; Haryanto, Sotianingsih ; Wiyatno, Ageng ; Soebandrio, Amin ; Myint, Khin Saw ; Ledermann, Jeremy P. ; Rosenberg, Ronald ; Powers, Ann M. ; Sasmono, R. Tedjo
• Description:
  Zika virus (ZIKV) JMB-185 strain was isolated from a febrile patient in Jambi, Indonesia in 2014. To understand its genetic characteristics, we performed whole genome sequencing using the Ion Torrent PGM platform on the supernatant of the first passage. The phylogenetic analysis showed that the isolate was not closely related to the Brazilian ZIKV associated with microcephaly or isolates from the recent Singapore Zika outbreak. Molecular evolution analysis indicated that JMB-185 strain may have been circulating in the Southeast Asia region, including Indonesia since 2000. We observed high nucleotide sequence identity between Indonesia, Thailand, Singapore, and American strains although unique amino acid substitutions were also observed. This report provides information on the genomic characteristics of Indonesian ZIKV which may be used for further studies.
• Subjects:
  Acid Substitution Amino Article Cluster Analysis Evolution, Molecular Genome Genome, Viral Indonesia Phylogeny RNA, Viral Sequence Analysis, DNA Sequence Homology Zika Virus Zika Virus Infection

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• Source:
  Virology. 510:248-251
• Pubmed ID:
  28755588
• Pubmed Central ID:
  PMC5747357
• Document Type:
  Journal Article
• Funding:
  CC999999/ImCDC/Intramural CDC HHSUnited States/ ; U18 CK000443/CK/NCEZID CDC HHSUnited States/
• Volume:
  510
• Collection(s):
  CDC Public Access
• Main Document Checksum:
  urn:sha256:ac9ef3c252fff6879fd1ea8944f00ba7c622dcb6bcdd72d5bf89cdaefdd36325
• Download URL:
  https://stacks.cdc.gov/view/cdc/50653/cdc_50653_DS1.pdf
• File Type:
  [PDF - 770.35 KB ]